Difference between revisions of "Part:BBa K1036000"
Tina Zhang (Talk | contribs) (→Description) |
Tina Zhang (Talk | contribs) (→Description) |
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=Description= | =Description= | ||
| − | This part is made up of a quorum sensing promoter and ''ndh'' gene with a LAV-tag. Under control of the quorum sensing promoter, ''ndh'' gene will express periodically as the promoter is periodically activated and repressed. Since the ''ndh'' gene is described detailed in Part: | + | <html> |
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| + | This part is made up of a quorum sensing promoter and ''ndh'' gene with a LAV-tag. Under control of the quorum sensing promoter, ''ndh'' gene will express periodically as the promoter is periodically activated and repressed. Since the ''ndh'' gene is described detailed in Part: <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036001">BBa_K1036001</a>, here is a detailed description of quorum sensing promoter. Firstly, we should be familiar with the quorum sensing mechanism, the ''luxI'' gene is at low expression level and produces LuxI protein that synthesizes a kind of acyl-homoserine lactone (AHL), which is a small molecule that can diffuse across the cell membrane and mediate intercellular coupling when it reaches the threshold as enough biomass accumulated. AHL will bind intracellular protein LuxR, which is also consecutively produced by ''luxR'' gene. The LuxR-AHL complex can activate the ''luxI'' promoter, and the positive feedback loop is built. However, since in this circuit the ''luxI'' gene behind the ''luxI'' promoter is changed into ''ndh'' gene (with LVA-tag), so it could only be activated by AHL produced by ''luxI'' gene on another plasmid, which contains Part: BBa_K1036003. | ||
| + | </body> | ||
| + | </html> | ||
=Usage and Biology= | =Usage and Biology= | ||
Revision as of 12:18, 26 September 2013
lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II
Description
This part is made up of a quorum sensing promoter and ''ndh'' gene with a LAV-tag. Under control of the quorum sensing promoter, ''ndh'' gene will express periodically as the promoter is periodically activated and repressed. Since the ''ndh'' gene is described detailed in Part: BBa_K1036001, here is a detailed description of quorum sensing promoter. Firstly, we should be familiar with the quorum sensing mechanism, the ''luxI'' gene is at low expression level and produces LuxI protein that synthesizes a kind of acyl-homoserine lactone (AHL), which is a small molecule that can diffuse across the cell membrane and mediate intercellular coupling when it reaches the threshold as enough biomass accumulated. AHL will bind intracellular protein LuxR, which is also consecutively produced by ''luxR'' gene. The LuxR-AHL complex can activate the ''luxI'' promoter, and the positive feedback loop is built. However, since in this circuit the ''luxI'' gene behind the ''luxI'' promoter is changed into ''ndh'' gene (with LVA-tag), so it could only be activated by AHL produced by ''luxI'' gene on another plasmid, which contains Part: BBa_K1036003.
Usage and Biology
Protocol
Ndh with LVA tag is formed as a composite by the ligation of quorum sensing promoter luxR via digestion and ligation.
Experimental Data
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2544
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1101