Difference between revisions of "Part:BBa K1150001"

Revision as of 12:13, 26 September 2013

VP16

Virus Protein 16 (VP16) is a transcription factor encoded by the UL48 gene of Herpes simplex virus-1 (HSV-1).

Through complex formation with cellular host factors VP16 can bind to a common regulatory element in the upstream promotor region of immediate-early genes [Weir, 2001]. Through the transactivating function of VP-16 these genes can then be expressed.

VP16 consists of 490 amino acids with two very important functional domains: a core domain in its central region which is necessary for the indirect DNA binding and a carboxy-terminal transactivation domain (Greaves and O’Hare, 1989; Triezenberg et al., 1988); see Fig.1. The transactivation domain (TAD) of VP16 can be fused to a DNA-binding domain (DBD) of another protein in order to gain expression of a desired target gene [Hirai et al., 2010].

Fig. 1: Domain structure of VP16. The different functional domains of VP16 are shown. Taken from Hirai et al., 2010.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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-VP16 is ...
+Virus Protein 16 (VP16) is a transcription factor encoded by the UL48 gene of Herpes simplex virus-1 (HSV-1).
+Through complex formation with cellular host factors VP16 can bind to a common regulatory element in the upstream promotor region of immediate-early genes [Weir, 2001]. Through the transactivating function of VP-16 these genes can then be expressed.
+VP16 consists of 490 amino acids with two very important functional domains: a core domain in its central region which is necessary for the indirect DNA binding and a carboxy-terminal transactivation domain (Greaves and O’Hare, 1989; Triezenberg et al., 1988); see Fig.1. The transactivation domain (TAD) of VP16 can be fused to a DNA-binding domain (DBD) of another protein in order to gain expression of a desired target gene [Hirai et al., 2010].
+'''Fig. 1:''' Domain structure of VP16. The different functional domains of VP16 are shown. Taken from Hirai et al., 2010.
 <!-- Add more about the biology of this part here
-===Usage and Biology===
+==Transactivation domain (TAD) of VP16==
+The TAD of VP16 is one of the most efficient TADs. It is widely fused to host transcription factors in order to increase their activity or, next to this, to other transcription factors to study the mechanism of gene regulation [Hirai et al., 2010].
+In our case the TAD of VP16 was cloned behind a double mutated Cas9 gene (which encodes for a non-cutting Cas9 protein with specific DNA binding properties) to activate our genes of interest. For more information  see [[BBa_K1150019]] and [[BBa_K1150020]].
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