Difference between revisions of "Part:BBa K1139100"

Revision as of 11:48, 26 September 2013

Adenylate isopentenyltransferase 4

In order to construct cytokinin production part, we focused on AtIPT, a plant enzyme which catalyzes the synthesis of cytokinin. The mechanisms of cytokinin synthesis are shown in Fig. 1 (Takei et al., 2001). We ordered a DNA sequence of AtIPT (AtIPT4) derived from A. thaliana and are now constructing the part including one of them.

Fig. 1. Mechanisms of cytokinin synthesis

AtIPT, a plant enzyme, catalyzes the synthesis of iPRMP from DMAPP and AMP. According to the previous research, DMAPP, and AMP are provided by authentic metabolism in E. coli. It is also expected in the report that E. coli also has the enzymes which catalyze the synthesis of iP or tZ from iPRMP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 328
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 61
1000
COMPATIBLE WITH RFC[1000]

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 [[Image:Titech2013_parts_K1139100_Fig1.jpg|thumb|center|500px|'''Fig. 1.''' Mechanisms of cytokinin synthesis]]
-AtIPT, a plant enzyme, catalyzes the synthesis of iPRMP from DMAPP and AMP. According to the previous research, DMAPP, and AMP are provided by authentic metabolism in <i>E. coli</i>.  It is also expected in the report that <i>E. coli</> also has the enzymes which catalyze the synthesis of iP or tZ from iPRMP.
+AtIPT, a plant enzyme, catalyzes the synthesis of iPRMP from DMAPP and AMP. According to the previous research, DMAPP, and AMP are provided by authentic metabolism in <i>E. coli</i>.  It is also expected in the report that <i>E. coli</i> also has the enzymes which catalyze the synthesis of iP or tZ from iPRMP.
 <!-- Add more about the biology of this part here