Difference between revisions of "Part:BBa K1036000:Design"

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===Design Notes===
 
===Design Notes===
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The ndh gene was amplifed from the genome of E. coli strain BL21(DE3) via PCR. And we know that the biobrick BBa_F2621 (position in plate: 2012-Plate 2-21F) is a quorum sensing promoter. We digested ''ndh'' with ''EcoR''I and ''Xba'' I, and 21F with ''EcoR''I and ''Spe''I. Then we construct ndh plasmid by using ligase.
+
The ''ndh'' gene was amplifed from the genome of ''E. coli'' strain BL21(DE3) via PCR. And we know that the biobrick BBa_F2621 (position in plate: 2012-Plate 2-21F) is a quorum sensing promoter. We digested ''ndh'' with ''EcoR''I and ''Xba'' I, and 21F with ''EcoR''I and ''Spe''I. Then we constructed ''ndh'' plasmid by ligation.
  
 
===Source===
 
===Source===

Revision as of 10:53, 26 September 2013


lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2544
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101


Design Notes

The ndh gene was amplifed from the genome of E. coli strain BL21(DE3) via PCR. And we know that the biobrick BBa_F2621 (position in plate: 2012-Plate 2-21F) is a quorum sensing promoter. We digested ndh with EcoRI and Xba I, and 21F with EcoRI and SpeI. Then we constructed ndh plasmid by ligation.

Source

The ndh gene was amplifed from the genome of E. coli strain BL21(DE3) via PCR.

References

1. Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)