Difference between revisions of "Part:BBa K1139021:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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<partinfo>BBa_K1139021 short</partinfo>
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how you used this part and how it worked out.
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We confirmed whether the release of M13 phage particle depends on the induction of <i>g2p</i> expression. We inserted <i>lux</i> promoter (activated by LuxR-3OC6HSL complex) upstream of <i>g2p</i>, as an inducible promoter.<br>
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[[Image:Titech2013_parts_K1139021_EX_Fig1.jpg|thumb|center|500px|'''Fig. 1.''' Flow chart of this experiment.]]
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Prepare <i>E. coli</i> surrounded by a red circle.  We add inducer (AHL) to the <i>E. coli</i>.  Phage is released by induction of AHL.  Spin the culture of the <i>E. coli</i>.  Decant the supernatant of the culture including phages to soft agar ( the agar which is easy to melt ).  Add lawn (<i>E. coli</i> containing pSB6A1-Ptet-<i>luxR</i> ) to the soft agar.  Mix them in soft agar.  Then, decant the soft agar to YT plate.<br>
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===1. Materials and Method===
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===2. Result===
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===Applications of BBa_K1139021===
 
===Applications of BBa_K1139021===

Revision as of 09:35, 26 September 2013

Plux-M13-Plac-GFP on pSB3

We confirmed whether the release of M13 phage particle depends on the induction of g2p expression. We inserted lux promoter (activated by LuxR-3OC6HSL complex) upstream of g2p, as an inducible promoter.

Fig. 1. Flow chart of this experiment.

Prepare E. coli surrounded by a red circle. We add inducer (AHL) to the E. coli. Phage is released by induction of AHL. Spin the culture of the E. coli. Decant the supernatant of the culture including phages to soft agar ( the agar which is easy to melt ). Add lawn (E. coli containing pSB6A1-Ptet-luxR ) to the soft agar. Mix them in soft agar. Then, decant the soft agar to YT plate.

1. Materials and Method

2. Result

Applications of BBa_K1139021

User Reviews

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