Difference between revisions of "Part:BBa K1119001:Design"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1119001 short</partinfo>
 
<partinfo>BBa_K1119001 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
No
+
This part is in RFC25 standard, which facilitate protein fusion in order to introduce other protein into mitochondria.
  
  
Line 13: Line 12:
 
===Source===
 
===Source===
  
−
Invitrogen: pCMV/myc/mito (http://tools.lifetechnologies.com/content/sfs/manuals/pshooter_pcmv_man.pdf)
+
The CDS sequence of MLS was cloned out from pCMV/myc/mito (Invitrogen, Carlsbard, CA) using PCR with primers that includes prefix and suffix in RFC25 standard. The original source of this CDS of MLS sequence is from that of subunit VIII of human cytochrome c oxidase. (Invitrogen, Carlsbard, CA)  
  
 
===References===
 
===References===

Revision as of 09:10, 26 September 2013

Mitochondrial Leader Sequence with RFC25 standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in RFC25 standard, which facilitate protein fusion in order to introduce other protein into mitochondria.


Source

The CDS sequence of MLS was cloned out from pCMV/myc/mito (Invitrogen, Carlsbard, CA) using PCR with primers that includes prefix and suffix in RFC25 standard. The original source of this CDS of MLS sequence is from that of subunit VIII of human cytochrome c oxidase. (Invitrogen, Carlsbard, CA)

References