Difference between revisions of "Part:BBa K1119008"

Revision as of 08:59, 26 September 2013

CMV promoter - GFP - hGH polyA tail

The CDS of MLS (BBa_K1119001) was assembled in frame with that of GFP reporter using Freiburg’s RFC25 format(BBa_K648013). The translation unit was driven by CMV promoter (BBa_K1119006) and terminated by hGH polyA signal (BBa_K404108).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 614
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 628
Illegal AgeI site found at 1369
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1772
Illegal BsaI.rc site found at 1274

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1119008 short</partinfo>
-Composite part for characterization of CMV promoter. CMV (Cytomegalovirus) promoter is a constitutive mammalian promoter that we used to express glyoxylate genes in mammalian cell. Our objective in characterizing this promoter is to test if the CMV promoter drives the expression of green fluorescence protein (GFP) in mammalian HEK293FT cell line.
+The CDS of MLS ([[Part:BBa_K1119001|BBa_K1119001]]) was assembled in frame with that of GFP reporter using Freiburg’s RFC25 format([[Part:BBa_K648013|BBa_K648013]]). The translation unit was driven by CMV promoter ([[Part:BBa_K1119006|BBa_K1119006]]) and terminated by hGH polyA signal ([[Part:BBa_K404108|BBa_K404108]]).
-CMV promoter characterization construct composes of CMV promoter, Green Fluorescence Protein (GFP) and hGH polyadenylation sequence (hGH pA) in pSB1C3 backbone.(BBa_K1119006 – BBa_K648013 – BBa_K404108 – pSB1C3)