Difference between revisions of "Part:BBa K1045014:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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This part was constructed using hybridization oligos for [[Part:BBa_K1045011|BBa_K1045011]]. The hybridization product corresponded to a DNA fragment harboring the sequence of [[Part:BBa_K1045011|BBa_K1045011]] cut with EcoRI and SpeI at the prefix and suffix sites. This fragment was ligated into [[Part:BBa_K1045013|BBa_K1045013]] in a prefixing composition.
+
This part was constructed using hybridization oligos for [[Part:BBa_K1045011|BBa_K1045011]]. The hybridization product corresponded to a DNA fragment harboring the sequence of [[Part:BBa_K1045011|BBa_K1045011]] cut with ''EcoRI'' and ''SpeI'' at the prefix and suffix sites. This fragment was ligated into [[Part:BBa_K1045013|BBa_K1045013]] in a prefixing composition.
  
−
This biobrick harbors a mutation in the suffix sequence. The mutated suffix sequence is: TACTAGTAACGGCCGCTGCAG. This eliminates the NotI restriction site. The plasmid might still be cut with SpeI and PstI.
+
This biobrick harbors a mutation in the suffix sequence. The mutated suffix sequence is: TACTAGTAACGGCCGCTGCAG. This eliminates the ''NotI'' restriction site. The plasmid might still be cut with ''SpeI'' and ''PstI''.
  
 
===Source===
 
===Source===

Revision as of 08:41, 26 September 2013

Promoter reverse - Promoter - DarR operator - GFP generator BBa_E0240


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
    Illegal NheI site found at 24
    Illegal NheI site found at 104
    Illegal NheI site found at 127
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
    Illegal AgeI site found at 963
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 825


Design Notes

This part was constructed using hybridization oligos for BBa_K1045011. The hybridization product corresponded to a DNA fragment harboring the sequence of BBa_K1045011 cut with EcoRI and SpeI at the prefix and suffix sites. This fragment was ligated into BBa_K1045013 in a prefixing composition.

This biobrick harbors a mutation in the suffix sequence. The mutated suffix sequence is: TACTAGTAACGGCCGCTGCAG. This eliminates the NotI restriction site. The plasmid might still be cut with SpeI and PstI.

Source

To be continued

References