Difference between revisions of "Part:BBa K1088011"

Revision as of 08:35, 26 September 2013

B. subtilis dxs (lac promoter without lac inhibitor)

This part has the dxs gene derived from B. subtilis (BBa_K1088000) controlled by the lac promoter and has a strong RBS. The part was build to increase the flow through the MEP pathway.

Fluorescence activated cell sorting (FACS) was used to examine the expression profile of a similar part (BBa_K1088008) with linker-GFP attached to the end of Dxs. The part was examined in the K-12 MG1655 (natural levels of LacI) and KG22 (overexpression of LacI from chromosome) strains. The experiment proved that overexpression of LacI is required for repression of the lac promoter (and therefore also control of the regulation). BBa_K1088027 is a similar part which overexpresses LacI from the part it self.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1279
Illegal EcoRI site found at 1936
Illegal PstI site found at 1337
Illegal PstI site found at 1783
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1279
Illegal EcoRI site found at 1936
Illegal PstI site found at 1337
Illegal PstI site found at 1783
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1279
Illegal EcoRI site found at 1936
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1279
Illegal EcoRI site found at 1936
Illegal PstI site found at 1337
Illegal PstI site found at 1783
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1279
Illegal EcoRI site found at 1936
Illegal PstI site found at 1337
Illegal PstI site found at 1783
Illegal NgoMIV site found at 1236
Illegal AgeI site found at 1129
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1078
Illegal SapI.rc site found at 1777

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1088011 short</partinfo>
-This part has the ''dxs'' gene derived from ''B. subtilis'' (BBa_K1088000) controlled by the common Lac promoter. Notice that overexpression of LacI is required for regulation of the lac promoter.
+This part has the ''dxs'' gene derived from ''B. subtilis'' (BBa_K1088000) controlled by the lac promoter and has a strong RBS. The part was build to increase the flow through the MEP pathway.
+Fluorescence activated cell sorting (FACS) was used to examine the expression profile of a similar part (BBa_K1088008) with linker-GFP attached to the end of Dxs. The part was examined in the K-12 MG1655 (natural levels of LacI) and KG22 (overexpression of LacI from chromosome) strains. The experiment proved that overexpression of LacI is required for repression of the lac promoter (and therefore also control of the regulation). BBa_K1088027 is a similar part which overexpresses LacI from the part it self.