Difference between revisions of "Part:BBa K1045017:Design"
(→Design Notes) |
(→Design Notes) |
||
| Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | DarR fused to the terminator were cut out from | + | DarR fused to the terminator were cut out from [[Part:BBa_K1045016|BBa_K1045016]] and ligated in a pre-fixing composition into [[Part:BBa_K1045015|BBa_K1045015]]. |
===Source=== | ===Source=== | ||
Revision as of 08:23, 26 September 2013
DarR reporter system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 806
Illegal NheI site found at 829
Illegal NheI site found at 909
Illegal NheI site found at 932
Illegal NotI site found at 718 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 731
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 860
Illegal AgeI site found at 168 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1630
Design Notes
DarR fused to the terminator were cut out from BBa_K1045016 and ligated in a pre-fixing composition into BBa_K1045015.
Source
To be continued