Difference between revisions of "Part:BBa K1139151"
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To characterize the function of the ''rm/lac'' hybrid promoter (<partinfo>BBa_K1139151</partinfo>), we constructed this part Prm/lac-GFP (<partinfo>BBa_K1139150</partinfo>) by inserting ''rm/lac'' promoter in front of a GFP coding sequence.<br>  
 
To characterize the function of the ''rm/lac'' hybrid promoter (<partinfo>BBa_K1139151</partinfo>), we constructed this part Prm/lac-GFP (<partinfo>BBa_K1139150</partinfo>) by inserting ''rm/lac'' promoter in front of a GFP coding sequence.<br>  
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By using the reporter cell that contains Prm/lac-GFP, we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, CI and IPTG (Fig. 1-B).
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By using the reporter cell that contains Prm/lac-GFP, we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, CI and IPTG (Fig. 1-B).<br>
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We confirmed that our new PRM/lac hybrid promoter was actually activated by CI through an induction assay (Fig. 1-C).
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[[Image:Titech2013_parts_K1139150_Fig1B.jpg|thumb|center|500px|'''Fig. 1-B.''' Comparison of N99 and JM2.300]]
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[[Image:Titech2013_parts_K1139150_Fig1B.jpg|thumb|center|500px|'''Fig. 1-B.''' Fluorescence intensity detected by flow cytometer]]
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[[Image:Titech2013_parts_K1139150_Fig1C.jpg|thumb|center|500px|'''Fig. 1-C.''' Comparison of N99 and JM2.300]]
  
 
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Revision as of 04:07, 26 September 2013

rm/lac hybrid promoter

1. Introduction
We newly developed rm/lac hybrid promoter which is activated by CI and repressed by LacI (Fig. 1-A).
To characterize the function of this hybrid promoter, we constructed a part Prm/lac-GFP (BBa_K1139150).

Fig. 1-A. Our new designed hybrid promoter

To characterize the function of the rm/lac hybrid promoter (BBa_K1139151), we constructed this part Prm/lac-GFP (BBa_K1139150) by inserting rm/lac promoter in front of a GFP coding sequence.
By using the reporter cell that contains Prm/lac-GFP, we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, CI and IPTG (Fig. 1-B).
We confirmed that our new PRM/lac hybrid promoter was actually activated by CI through an induction assay (Fig. 1-C).

Fig. 1-B. Fluorescence intensity detected by flow cytometer
Fig. 1-C. Comparison of N99 and JM2.300

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]