Difference between revisions of "Part:BBa K1080006:Design"
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===Design Notes=== | ===Design Notes=== | ||
Incorporated sequence overlap for Gibson assembly and no GC rich regions or restriction sites within sequence. | Incorporated sequence overlap for Gibson assembly and no GC rich regions or restriction sites within sequence. | ||
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+ | <b> Crystal Structure</b> | ||
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+ | http://pfam.sanger.ac.uk/structure/getimage?id=2plt | ||
<b>Amino acid sequence</b> | <b>Amino acid sequence</b> |
Revision as of 03:05, 26 September 2013
Plastocyanin
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 100
Illegal BsaI.rc site found at 223
Design Notes
Incorporated sequence overlap for Gibson assembly and no GC rich regions or restriction sites within sequence.
Crystal Structure
http://pfam.sanger.ac.uk/structure/getimage?id=2plt
Amino acid sequence
MATVKLGADS GALEFVPKTL TIKSGETVNF VNNAGFPHNI VFDEDAIPSG VNADAISRDD
YLNAPGETYS VKLTAAGEYG YYCEPHQGAG MVGKIIVQ
References and documentation are available. Please note the modified algorithm for extinction coefficient.
Number of amino acids: 98
Molecular weight: 10339.5
Theoretical pI: 4.57
Amino acid composition: Ala (A) 11 11.2% Arg (R) 1 1.0% Asn (N) 6 6.1% Asp (D) 6 6.1% Cys (C) 1 1.0% Gln (Q) 2 2.0% Glu (E) 6 6.1% Gly (G) 11 11.2% His (H) 2 2.0% Ile (I) 6 6.1% Leu (L) 5 5.1% Lys (K) 5 5.1% Met (M) 2 2.0% Phe (F) 4 4.1% Pro (P) 5 5.1% Ser (S) 5 5.1% Thr (T) 6 6.1% Trp (W) 0 0.0% Tyr (Y) 5 5.1% Val (V) 9 9.2% Pyl (O) 0 0.0% Sec (U) 0 0.0%
(B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 12
Total number of positively charged residues (Arg + Lys): 6
Atomic composition:
Carbon C 460 Hydrogen H 708 Nitrogen N 118 Oxygen O 147 Sulfur S 3
Formula: C460H708N118O147S3 Total number of atoms: 1436
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 7450 Abs 0.1% (=1 g/l) 0.721, assuming all pairs of Cys residues form cystines
Ext. coefficient 7450
Abs 0.1% (=1 g/l) 0.721, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is:
30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 18.58 This classifies the protein as stable.
Aliphatic index: 81.63
Grand average of hydropathicity (GRAVY): -0.065
Source
From genome of Chlamydomonas reinhardtii