Difference between revisions of "Part:BBa K1166002"

Revision as of 21:39, 25 September 2013

HlyA-tag+Secretion system

This is a device that allows a protein to be secreted by means of the alpha-hemolysin secretion system in E. coli. It’s designed so that the only thing that you have to do is to assemble your protein part in the device via the biofusion standard (BBF RFC 23), (cut the device with EcoRI and XbaI, cut your protein part with EcoRI and SpeI, mix & ligate). This procedure will leave your part in frame with a signal peptide.

Note: It’s important that your part doesn’t contain a stop codon nor a terminator since your protein will be fused at the C-terminus.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1550
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1489
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1324
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1306

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1166002 short</partinfo>
-This is a device that allows a protein to be secreted by means of the alpha-hemolysin secretion system in ''E. coli''. It’s designed so that the only thing that you have to do is to assemble your protein part in the device via the biofusion standard (BBF RFC 23), (Cut the device with EcoRI and XbaI, cut your protein part with EcoRI and SpeI, mix & ligate). This procedure will leave your part in frame with a signal peptide.
+This is a device that allows a protein to be secreted by means of the alpha-hemolysin secretion system in ''E. coli''. It’s designed so that the only thing that you have to do is to assemble your protein part in the device via the biofusion standard (BBF RFC 23), (cut the device with EcoRI and XbaI, cut your protein part with EcoRI and SpeI, mix & ligate). This procedure will leave your part in frame with a signal peptide.
 '''Note:''' It’s important that your part doesn’t contain a stop codon nor a terminator since your protein will be fused at the C-terminus.