Difference between revisions of "Part:BBa K1166002"
(Created page with "This is a device that allows a protein to be secreted by means of the alpha-hemolysin secretion system in E. coli. It’s designed so that the only thing that you have to do is ...") |
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It’s designed so that the only thing that you have to do is to assemble your protein part in the device via the biofusion standard (BBF RFC 23), (Cut the device with EcoRI and XbaI, cut your protein part with EcoRI and SpeI, mix & ligate). This procedure will leave your part in-frame with a signal peptide. | It’s designed so that the only thing that you have to do is to assemble your protein part in the device via the biofusion standard (BBF RFC 23), (Cut the device with EcoRI and XbaI, cut your protein part with EcoRI and SpeI, mix & ligate). This procedure will leave your part in-frame with a signal peptide. | ||
| − | Note: It’s important that your part doesn’t contain a stop codon nor a terminator since your protein will be fused at the C- | + | Note: It’s important that your part doesn’t contain a stop codon nor a terminator since your protein will be fused at the C-terminus. |
Revision as of 21:28, 25 September 2013
This is a device that allows a protein to be secreted by means of the alpha-hemolysin secretion system in E. coli.
It’s designed so that the only thing that you have to do is to assemble your protein part in the device via the biofusion standard (BBF RFC 23), (Cut the device with EcoRI and XbaI, cut your protein part with EcoRI and SpeI, mix & ligate). This procedure will leave your part in-frame with a signal peptide.
Note: It’s important that your part doesn’t contain a stop codon nor a terminator since your protein will be fused at the C-terminus.