Difference between revisions of "Part:BBa K1140006"
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<partinfo>BBa_K1140006 short</partinfo> | <partinfo>BBa_K1140006 short</partinfo> | ||
| − | mCherry | + | Transcription of mCherry is under the regulation of a pTet promoter. Translation is also regulated by a synthetic RNA thermometer[1] that allows translation only over 37 °C. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 20:56, 25 September 2013
pTet + 37 oC RNA thermometer + mCherry (LVA)
Transcription of mCherry is under the regulation of a pTet promoter. Translation is also regulated by a synthetic RNA thermometer[1] that allows translation only over 37 °C.
Usage and Biology
RNA thermometers (RNAT) are temperature sensing RNA sequences that regulate translation by preventing the ribosome from binding the transcript until higher temperatures shift it to an open structure. Several naturally occurring RNA thermometers have been described and synthetic sequences that emulate them have been designed and proved to regulate genetic expression at different temperature ranges. The RNAT herein implemented, designed and dubbed u6 by Neupert et al. [1], is a synthetic sequence that allows translation only at temperatures over 37 °C. This part can be incorporated into a genetic circuit to integrate transcriptional and post-transcriptional regulation, through the Tet Repressor and u6-RNAT, respectively.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 63
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 676
Illegal AgeI site found at 788 - 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
| direction | Forward |
| function | Integrated Transcriptional and Translational regulation |
| protein | mCherry |
| switch_point | 37 celsius degrees |
| tag | LVA |