Difference between revisions of "Part:BBa K1088016"

Revision as of 18:20, 25 September 2013

HRT2 prenyltransferase from Hevea Brasilianis (ara promoter with araC: arabinose inducible)

This biobrick expresses the Hevea brasiliensis rubber prenyltransferase when induced with arabinose under glucose scarce conditions. Arabinose binds the ara promoter regulator, AraC, and AraC bound arabinose activates the transcription of the ara promoter

Using Northern blot technique it was proved that the construct in E. coli K-12 MG1655 doesn't express the prenyltransferase when grown without arabinose. Upon addition of arabinose to the media prenyltranferase mRNA was detected. See experince for more details.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1102
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1042
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This biobrick expresses the ''Hevea brasiliensis'' rubber prenyltransferase when induced with arabinose under glucose scarce conditions. Arabinose binds the ara promoter regulator, AraC, and AraC bound arabinose activates the transcription of the ara promoter
-Using Northern blot technique it was proved that the constructs in ''E. coli'' K-12 MG1655 doesn't express the prenyltransferase when grown without arabinose. Upon addition of arabinose to the media prenyltranferase mRNA was detected. See experince for more details.
+Using Northern blot technique it was proved that the construct in ''E. coli'' K-12 MG1655 doesn't express the prenyltransferase when grown without arabinose. Upon addition of arabinose to the media prenyltranferase mRNA was detected. See experince for more details.
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