Difference between revisions of "Part:BBa K1088014"

 
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<partinfo>BBa_K1088014 short</partinfo>
 
<partinfo>BBa_K1088014 short</partinfo>
  
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This system allows IPTG controlled expression of the Dxs enzyme isolated from B. sub.  
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This part consist of the ''dxs'' gene derived from ''E. coli'' under the control of the lac promoter and has a strong RBS.
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To repress expression from the lac promoter, the ''lacI:LVA'' gene (under a constitutive promoter, with a strong RBS and a efficient terminator) was placed counter-clockwise to the ''dxs'' gene. The repression can be relieved with addition of IPTG, which binds and inhibits the function of LacI:LVA.
  
 
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Revision as of 18:09, 25 September 2013

E. coli dxs (lac promoter with lac inhibitor: IPTG inducible)

This part consist of the dxs gene derived from E. coli under the control of the lac promoter and has a strong RBS.

To repress expression from the lac promoter, the lacI:LVA gene (under a constitutive promoter, with a strong RBS and a efficient terminator) was placed counter-clockwise to the dxs gene. The repression can be relieved with addition of IPTG, which binds and inhibits the function of LacI:LVA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2158