Difference between revisions of "Part:BBa K1088010"
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<partinfo>BBa_K1088010 short</partinfo> | <partinfo>BBa_K1088010 short</partinfo> | ||
| − | This | + | This part consist of the dxs gene derived from ''E. coli'' fused to GFP at the translational level with a 10 AA linker between the proteins. The reporter fusion is under the control of the lac promoter and has a strong RBS. |
| − | + | To repress expression from the lac promoter, the lacI:LVA (gene under a constitutive promoter, with a strong RBS and a efficient terminator) was placed counter-clockwise to the reporter fusion. The repression can be relieved with addition of IPTG, which binds and inhibits the function of LacI:LVA. | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 17:41, 25 September 2013
E. coli dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG inducib
This part consist of the dxs gene derived from E. coli fused to GFP at the translational level with a 10 AA linker between the proteins. The reporter fusion is under the control of the lac promoter and has a strong RBS. To repress expression from the lac promoter, the lacI:LVA (gene under a constitutive promoter, with a strong RBS and a efficient terminator) was placed counter-clockwise to the reporter fusion. The repression can be relieved with addition of IPTG, which binds and inhibits the function of LacI:LVA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3937
Illegal SapI.rc site found at 2127