Difference between revisions of "Part:BBa K1164004"
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===Usage and Biology===
 
===Usage and Biology===
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Test
  
 
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Revision as of 16:37, 25 September 2013

pTRELX: Modified Gal1 promoter; gal4O replaced w/ tetO; repressable via lacO

This is a modified version of the native Gal1 promoter found in S.cerevisiae. The four gal4 binding domains present in the native promoter have been replaced by four tetO sites. This promoter may be activated by regulatory activators that are able to bind to tetO. In addition, a lacO operator has been introduced downstream of the TATA box, allowing for repression of gene expression by lacI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 465
    Illegal BamHI site found at 553
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 81
  • 1000
    COMPATIBLE WITH RFC[1000]