Difference between revisions of "Part:BBa K1139020"

Revision as of 16:17, 25 September 2013

PlacIq-M13-Plac-GFP on pSB3

PlacIQ is a constitutive promoter.

We confirmed that M13 genome with two modifications (PlacIq BBa_I14032) related to our design kept plaque forming activity. One is replacement of the promoter for g2p protein with a constitutive promoter, PlacIq(BBa_I14032). The other is accommodation of pSB3K3 backbone. Even though the plasmid has two different types of replication origins, M13 origin and pSB3 origin, this plasmid (BBa_K1139020) formed plaque. In contrast, construction intermediates without a promoter for g2p coding sequence (Promoterless-M13 + Plac, Promoterless-M13 + PlacGFP BBa_K1139022) could not form plaque.

GFP expression is regulated by LacI repressor.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 145
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 6071
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 7335

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 PlacIQ is a constitutive promoter.
+We confirmed that M13 genome with two modifications (PlacIq [https://parts.igem.org/Part:BBa_I14032 BBa_I14032]) related to our design kept plaque forming activity.  One is replacement of the promoter for g2p protein with a constitutive promoter, PlacIq([https://parts.igem.org/Part:BBa_I14032 BBa_I14032]).  The other is accommodation of pSB3K3 backbone. Even though the plasmid has two different types of replication origins, M13 origin and pSB3 origin, this plasmid ([https://parts.igem.org/Part:BBa_1139020 BBa_K1139020]) formed plaque.  In contrast, construction intermediates without a promoter for g2p coding sequence (Promoterless-M13 + Plac, Promoterless-M13 + PlacGFP [https://parts.igem.org/Part:BBa_1139022 BBa_K1139022]) could not form plaque.
 <i>GFP</i> expression is regulated by LacI repressor.