Difference between revisions of "Part:BBa K1045005:Design"
(→Design Notes) |
|||
| Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | This part was amplified from Bacillus subtilis DNA and ligated into EcoRI and PstI digested '''pSB1C3''' backbone. | + | This part was amplified from ''Bacillus subtilis'' genomic DNA and ligated into ''EcoRI'' and ''PstI'' digested '''pSB1C3''' backbone. |
===Source=== | ===Source=== | ||
| − | + | ||
===References=== | ===References=== | ||
Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an c-di-AMP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965 | Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an c-di-AMP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965 | ||
Revision as of 13:03, 25 September 2013
YdaO Riboswitch with native RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was amplified from Bacillus subtilis genomic DNA and ligated into EcoRI and PstI digested pSB1C3 backbone.
Source
References
Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an c-di-AMP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965