Difference between revisions of "Part:BBa K1045013:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | For cloning of [[Part:BBa_K1045013|BBa_K1045013]], first, oligos for [[Part:BBa_K1045012|BBa_K1045012]] were hybridized. The hybridization oligos were designed such that their double-stranded hybridization product would correspond to the sequence of [[Part:BBa_K1045012|BBa_K1045012]] cut with EcoRI and SpeI at the prefix and suffix sites. Second, this hybridization product was ligated in front of the GFP generator [[Part:BBa_E0240|BBa_E0240]] previously cut with EcoRI and XbaI. | + | For cloning of [[Part:BBa_K1045013|BBa_K1045013]], first, oligos for [[Part:BBa_K1045012|BBa_K1045012]] were hybridized. The hybridization oligos were designed such that their double-stranded hybridization product would correspond to the sequence of [[Part:BBa_K1045012|BBa_K1045012]] cut with ''EcoRI'' and ''SpeI'' at the prefix and suffix sites. Second, this hybridization product was ligated in front of the GFP generator [[Part:BBa_E0240|BBa_E0240]] previously cut with ''EcoRI'' and ''XbaI''. |
===Source=== | ===Source=== | ||
Revision as of 12:54, 25 September 2013
Promoter - DarR operator - GFP generator BBa_E0240
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 728
Design Notes
For cloning of BBa_K1045013, first, oligos for BBa_K1045012 were hybridized. The hybridization oligos were designed such that their double-stranded hybridization product would correspond to the sequence of BBa_K1045012 cut with EcoRI and SpeI at the prefix and suffix sites. Second, this hybridization product was ligated in front of the GFP generator BBa_E0240 previously cut with EcoRI and XbaI.
Source
To be continued