Difference between revisions of "Part:BBa I13543"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_I13543 short</partinfo> | <partinfo>BBa_I13543 short</partinfo> | ||
| − | + | -- No description -- | |
| − | -- No description -- | + | |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | Can also be used as an RFP only control, to compare to measurements such as in pSB1A10. Transcription must be induced off of Pbad, so this part should be used in strain CW2553/pJat8 and induced at a high arabinose concentration such as 3e-4%. | |
| − | + | <br><br> | |
| + | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_I13543 SequenceAndFeatures</partinfo> | <partinfo>BBa_I13543 SequenceAndFeatures</partinfo> | ||
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| + | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_I13543 parameters</partinfo> | <partinfo>BBa_I13543 parameters</partinfo> | ||
| + | <!-- --> | ||
Latest revision as of 02:57, 18 July 2006
Intermediate in two promoter screening plasmid
-- No description --
Usage and Biology
Can also be used as an RFP only control, to compare to measurements such as in pSB1A10. Transcription must be induced off of Pbad, so this part should be used in strain CW2553/pJat8 and induced at a high arabinose concentration such as 3e-4%.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1325
Illegal BamHI site found at 1144
Illegal XhoI site found at 1303 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1910
Illegal AgeI site found at 2022 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961