Difference between revisions of "Part:BBa I13540"
 
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<partinfo>BBa_I13540 short</partinfo>
 
<partinfo>BBa_I13540 short</partinfo>
  
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Can also be used as a GFP only control, to compare to measurements such as in pSB1A10.  Pbad must be induced to high transcription, so I13540 should be used in strain CW2553/pJat8 with high arabinose induction such as 3e-3% (Horazdovsky BF and Hogg RW. Genetic reconstitution of the high-affinity L-arabinose transport system. J Bacteriol 1989 Jun; 171(6) 3053-9. pmid:2656640.)
 
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===Usage and Biology===
 
===Usage and Biology===
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Can also be used as a GFP only control, to compare to measurements such as in pSB1A10. Transcription must be induced off of Pbad, so this part should be used in strain CW2553/pJat8 and induced at a high arabinose concentration such as 3e-4%.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 02:57, 18 July 2006

Intermediate in two promoter screening plasmid

Usage and Biology

Can also be used as a GFP only control, to compare to measurements such as in pSB1A10. Transcription must be induced off of Pbad, so this part should be used in strain CW2553/pJat8 and induced at a high arabinose concentration such as 3e-4%.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1880
    Illegal SapI site found at 961