Difference between revisions of "Part:BBa K1045005:Design"

(Design Notes)
(Design Notes)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
This part was amplified from Bacillus subtilis DNA and ligated into EcoRI and PstI digested '''pSBC13''' backbone.
+
This part was amplified from Bacillus subtilis DNA and ligated into EcoRI and PstI digested '''pSB1C3''' backbone.
  
 
===Source===
 
===Source===

Revision as of 11:36, 25 September 2013

YdaO Riboswitch with native RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis DNA and ligated into EcoRI and PstI digested pSB1C3 backbone.

Source

To be continued

References

Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an c-di-AMP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965