Difference between revisions of "Part:BBa K1045004:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 7: Line 7:
 
===Design Notes===
 
===Design Notes===
  
−
This part was amplified from Bacillus subtilis DNA and ligated into EcoRI and PstI digested [[Part:pSBC13|pSBC13]] backbone.
+
This part was amplified from Bacillus subtilis DNA and ligated into EcoRI and PstI digested '''pSBC13''' backbone.
  
 
===Source===
 
===Source===

Revision as of 11:32, 25 September 2013

YdaO Riboswitch with native Promoter and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis DNA and ligated into EcoRI and PstI digested pSBC13 backbone.

Source

To be continued

References

Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an c-di-AMP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965