Difference between revisions of "Part:BBa K1080001:Design"

Latest revision as of 10:13, 25 September 2013

ChlH

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1928
Illegal BglII site found at 2234
Illegal BglII site found at 3620
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 486
Illegal NgoMIV site found at 2152
Illegal AgeI site found at 1132
Illegal AgeI site found at 2650
Illegal AgeI site found at 2704
Illegal AgeI site found at 2923
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2254
Illegal BsaI.rc site found at 3049
Illegal SapI.rc site found at 2979

Design Notes

Amino Acid Sequence:

MCNVATGPRP PMTTFTGGNK GPAKQQVSLD LRDDGAGMFT STSPEMRRVV PDDVKGRVKV
KVVYVVLEAQ YQSAISAAVK NINAKNSKVC FEVVGYLLEE LRDQKNLDML KEDVASANIF
IGSLIFIEEL AEKIVEAVSP LREKLDACLI FPSMPAVMKL NKLGTFSMAQ LGQSKSVFSE
FIKSARKNND NFEEGLLKLV RTLPKVLKYL PSDKAQDAKN FVNSLQYWLG GNSDNLENLL
LNTVSNYVPA LKGVDFSVAE PTAYPDVGIW HPLASGMYED LKEYLNWYDT RKDMVFAKDA
PVIGLVLQRS HLVTGDEGHY SGVVAELESR GAKVIPVFAG GLDFSAPVKK FFYDPLGSGR
TFVDTVVSLT GFALVGGPAR QDAPKAIEAL KNLNVPYLVS LPLVFQTTEE WLDSELGVHP
VQVALQVALP ELDGAMEPIV FAGRDSNTGK SHSLPDRIAS LCARAVNWAN LRKKRNAEKK
LAVTVFSFPP DKGNVGTAAY LNVFGSIYRV LKNLQREGYD VGALPPSEED LIQSVLTQKE
AKFNSTDLHI AYKMKVDEYQ KLCPYAEALE ENWGKPPGTL NTNGQELLVY GRQYGNVFIG
VQPTFGYEGD PMRLLFSKSA SPHHGFAAYY TFLEKIFKAD AVLHFGTHGS LEFMPGKQVG
MSGVCYPDSL IGTIPNLYYY AANNPSEATI AKRRSYANTI SYLTPPAENA GLYKGLKELK
ELISSYQGMR ESGRAEQICA TIIETAKLCN LDRDVTLPDA DAKDLTMDMR DSVVGQVYRK
LMEIESRLLP CGLHVVGCPP TAEEAVATLV NIAELDRPDN NPPIKGMPGI LARAIGRDIE
SIYSGNNKGV LADVDQLQRI TEASRTCVRE FVKDRTGLNG RIGTNWITNL LKFTGFYVDP
WVRGLQNGEF ASANREELIT LFNYLEFCLT QVVKDNELGA LVEALNGQYV EPGPGGDPIR
NPNVLPTGKN IHALDPQSIP TQAALKSARL VVDRLLDRER DNNGGKYPET IALVLWGTDN
IKTYGESLAQ VMMMVGVKPV ADALGRVNKL EVIPLEELGR PRVDVVVNCS GVFRDLFVNQ
AVENSSWSDE SQLQEMYLKR KSYAFNSDRP GAGGEMQRDV FETAMKTVDV TFQNLDSSEI
SLTDVSHYFD SDPTKLVASL RNDGRTPNAY IADTTTANAQ VRTLGETVRL DARTKLLNPK
WYEGMLASGY EGVREIQKRM TNTMGWSATS GMVDNWVYDE ANSTFIEDAA MAERLMNTNP
NSFRKLVATF LEANGRGYWD AKPEQLERLR QLYMDVEDKI EGVE

References and documentation are available. Please note the modified algorithm for extinction coefficient.

Number of amino acids: 1364

Molecular weight: 150738.5

Theoretical pI: 5.39

Amino acid composition: Ala (A) 114 8.4% Arg (R) 69 5.1% Asn (N) 79 5.8% Asp (D) 81 5.9% Cys (C) 13 1.0% Gln (Q) 46 3.4% Glu (E) 91 6.7% Gly (G) 100 7.3% His (H) 14 1.0% Ile (I) 51 3.7% Leu (L) 141 10.3% Lys (K) 79 5.8% Met (M) 35 2.6% Phe (F) 50 3.7% Pro (P) 70 5.1% Ser (S) 84 6.2% Thr (T) 69 5.1% Trp (W) 14 1.0% Tyr (Y) 49 3.6% Val (V) 115 8.4% Pyl (O) 0 0.0% Sec (U) 0 0.0%

(B)   0	  0.0%
(Z)   0	  0.0%
(X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 172 Total number of positively charged residues (Arg + Lys): 148

Atomic composition:

Carbon C 6703 Hydrogen H 10581 Nitrogen N 1817 Oxygen O 2036 Sulfur S 48

Formula: C6703H10581N1817O2036S48 Total number of atoms: 21185

Extinction coefficients:

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 150760 Abs 0.1% (=1 g/l) 1.000, assuming all pairs of Cys residues form cystines

Ext. coefficient 150010 Abs 0.1% (=1 g/l) 0.995, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is:

                            30 hours (mammalian reticulocytes, in vitro).
                           >20 hours (yeast, in vivo).
                           >10 hours (Escherichia coli, in vivo).

Instability index:

The instability index (II) is computed to be 32.83 This classifies the protein as stable.

Aliphatic index: 87.71

Grand average of hydropathicity (GRAVY): -0.260

Source

Chlamydomonas reinhardtii

@@ Line 8: / Line 8: @@
 ===Design Notes===
-CR-ChlH sequence with His Tag in pET15b. Sequence from Translation start site.
+<b> Amino Acid Sequence:</b>
-Note 8 PstI sites CTGCAG (785, 1031, 1385, 1652, 2681, 2825, 3458, 3569) No EcoRI or XbaI sites. 1 SpeI (ACTAGT) (4226) site 23 bp after stop codon.
+<FONT FACE="courier">MCNVATGPRP PMTTFTGGNK GPAKQQVSLD LRDDGAGMFT STSPEMRRVV PDDVKGRVKV<br> KVVYVVLEAQ YQSAISAAVK NINAKNSKVC FEVVGYLLEE LRDQKNLDML KEDVASANIF<br> IGSLIFIEEL AEKIVEAVSP LREKLDACLI FPSMPAVMKL NKLGTFSMAQ LGQSKSVFSE<br> FIKSARKNND NFEEGLLKLV RTLPKVLKYL PSDKAQDAKN FVNSLQYWLG GNSDNLENLL<br> LNTVSNYVPA LKGVDFSVAE PTAYPDVGIW HPLASGMYED LKEYLNWYDT RKDMVFAKDA<br>  PVIGLVLQRS HLVTGDEGHY SGVVAELESR GAKVIPVFAG GLDFSAPVKK FFYDPLGSGR<br> TFVDTVVSLT GFALVGGPAR QDAPKAIEAL KNLNVPYLVS LPLVFQTTEE WLDSELGVHP<br> VQVALQVALP ELDGAMEPIV FAGRDSNTGK SHSLPDRIAS LCARAVNWAN LRKKRNAEKK<br> LAVTVFSFPP DKGNVGTAAY LNVFGSIYRV LKNLQREGYD VGALPPSEED LIQSVLTQKE<br> AKFNSTDLHI AYKMKVDEYQ KLCPYAEALE ENWGKPPGTL NTNGQELLVY GRQYGNVFIG<br>  VQPTFGYEGD PMRLLFSKSA SPHHGFAAYY TFLEKIFKAD AVLHFGTHGS LEFMPGKQVG<br> MSGVCYPDSL IGTIPNLYYY AANNPSEATI AKRRSYANTI SYLTPPAENA GLYKGLKELK<br> ELISSYQGMR ESGRAEQICA TIIETAKLCN LDRDVTLPDA DAKDLTMDMR DSVVGQVYRK<br> LMEIESRLLP CGLHVVGCPP TAEEAVATLV NIAELDRPDN NPPIKGMPGI LARAIGRDIE<br> SIYSGNNKGV LADVDQLQRI TEASRTCVRE FVKDRTGLNG RIGTNWITNL LKFTGFYVDP<br> WVRGLQNGEF ASANREELIT LFNYLEFCLT QVVKDNELGA LVEALNGQYV EPGPGGDPIR<br> NPNVLPTGKN IHALDPQSIP TQAALKSARL VVDRLLDRER DNNGGKYPET IALVLWGTDN<br> IKTYGESLAQ VMMMVGVKPV ADALGRVNKL EVIPLEELGR PRVDVVVNCS GVFRDLFVNQ<br> AVENSSWSDE SQLQEMYLKR KSYAFNSDRP GAGGEMQRDV FETAMKTVDV TFQNLDSSEI<br> SLTDVSHYFD SDPTKLVASL RNDGRTPNAY IADTTTANAQ VRTLGETVRL DARTKLLNPK<br> WYEGMLASGY EGVREIQKRM TNTMGWSATS GMVDNWVYDE ANSTFIEDAA MAERLMNTNP<br> NSFRKLVATF LEANGRGYWD AKPEQLERLR QLYMDVEDKI EGVE </FONT>
-<FONT FACE="courier">ATGCGGGGTTCTCATCATCATCATCATCATGGTATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCATCCCTTCACCGCGTGCAATGTGGCGACTGGACCCCGGCCGCCCATGACCACCTTCACCGGTGGCAACAAGGGCCCTGCTAAGCAGCAGGTGTCGCTGGATCTGCGCGACGAGGGCGCTGGCATGTTCACCAGCACCAGCCCGGAGATGCGCCGTGTCGTCCCTGACGATGTGAAGGGTCGCGTTAAGGTGAAGGTTGTGTACGTGGTGCTGGAGGCCCAGTACCAGTCGGCCATCAGCGCTGCGGTGAAGAACATCAACGCCAAGAACTCCAAGGTGTGCTTCGAGGTGGTGGGCTACCTGCTGGAGGAGCTGCGTGACCAGAAGAACCTCGATATGCTCAAGGAGGATGTGGCCTCTGCCAACATCTTCATCGGCTCGCTCATCTTCATTGAGGAGCTTGCCGAGAAGATTGTGGAGGCGGTGAGCCCCCTGCGCGAGAAGCTGGACGCGTGCCTGATCTTCCCGTCCATGCCGGCGGTCATGAAGCTGAACAAGCTGGGCACGTTTTCGATGGCTCAGCTGGGCCAGTCGAAGTCGGTGTTCTCGGAGTTCATCAAGTCTGCTCGCAAGAACAACGACAACTTCGAGGAGGGCTTGCTGAAGCTGGTGCGCACCCTGCCTAAGGTGCTGAAGTATCTGCCCTCGGACAAGGCGCAGGACGCCAAGAACTTCGTGAACAGCCTGCAGTACTGGCTGGGCGGTAACTCGGACAACCTGGAGAACCTGCTGCTGAACACCGTCAGCAACTACGTGCCCGCTCTGAAGGGCGTGGACTTCAGCGTGGCTGAGCCCACCGCCTACCCCGATGTGGGTATCTGGCACCCTCTGGCCTCGGGCATGTACGAGGACCTGAAGGAGTACCTGAACTGGTACGACACCCGCAAGGACATGGTCTTCGCCAAGGACGCCCCCGTCATTGGCCTGGTGCTGCAGCGCTCGCACCTGGTGACTGGCGATGAGGGCCACTACAGCGGCGTGGTCGCTGAGCTGGAGAGCCGCGGTGCTAAGGTCATCCCCGTCTTTGCCGGTGGCCTGGACTTCTCCGCCCCCGTCAAGAAGTTCTTCTACGACCCCCTGGGCTCTGGCCGCACGTTCGTGGACACCGTTGTGTCGCTGACCGGCTTCGCGCTGGTGGGCGGCCCCGCGCGCCAGGACGCGCCGAAGGCCATTGAGGCGCTGAAGAACCTGAACGTGCCCTACCTGGTGTCGCTGCCGCTGGTGTTCCAGACCACTGAGGAGTGGCTGGACAGCGAGCTGGGCGTGCACCCCGTCCAGGTGGCTCTGCAGGTTGCCCTGCCCGAGCTGGATGGTGCCATGGAGCCCATCGTGTTCGCTGGCCGTGACTCGAACACCGGCAAGTCGCACTCGCTGCCCGACCGCATCGCTTCGCTGTGCGCTCGCGCCGTGAACTGGGCCAACCTGCGCAAGAAGCGCAACGCCGAGAAGAAGCTGGCCGTCACCGTGTTCAGCTTCCCCCCTGACAAGGGCAACGTCGGCACTGCCGCCTACCTGAACGTGTTCGGCTCCATCTACCGCGTGCTGAAGAACCTGCAGCGCGAGGGCTACGACGTGGGCGCCCTGCCGCCCTCGGAGGAGGATCTGATCCAGTCGGTGCTGACCCAGAAGGAGGCCAAGTTCAACTCGACCGACCTGCACATCGCCTACAAGATGAAGGTGGACGAGTACCAGAAGCTGTGCCCTTACGCCGAGGCGCTGGAGGAGAACTGGGGCAAGCCCCCCGGCACCCTGAACACCAACGGCCAGGAGCTGCTGGTGTACGGCCGCCAGTACGGCAACGTCTTCATCGGCGTGCAGCCCACCTTCGGCTACGAGGGCGACCCGATGCGCCTGCTGTTCTCGAAGTCGGCCAGCCCCCACCACGGCTTCGCCGCCTACTACACCTTCCTGGAGAAGATCTTCAAGGCCGACGCCGTGCTGCACTTCGGCACCCACGGCTCGCTGGAGTTCATGCCCGGCAAGCAGGTCGGCATGTCGGGTGTGTGCTACCCCGACTCGCTGATCGGCACCATCCCCAACCTCTACTACTACGCCGCCAACAACCCGTCTGAGGCCACCATCGCCAAGCGCCGCTCGTACGCCAACACCATTTCGTACCTGACGCCGCCTGCCGAGAACGCCGGCCTGTACAAGGGCCTGAAGGAGCTGAAGGAGCTGATCAGCTCGTACCAGGGCATGCGTGAGTCTGGCCGCGCCGAGCAGATCTGCGCCACCATCATTGAGACCGCCAAGCTGTGCAACCTGGACCGCGACGTGACCCTGCCCGACGCTGACGCCAAGGACCTGACCATGGACATGCGCGACAGCGTTGTGGGCCAGGTGTACCGCAAGCTGATGGAGATTGAGTCCCGCCTGCTGCCCTGCGGCCTGCACGTGGTGGGCTGCCCGCCCACCGCCGAGGAGGCCGTGGCCACCCTGGTCAACATCGCTGAGCTGGACCGCCCGGACAACAACCCCCCCATCAAGGGCATGCCCGGCATCCTGGCCCGCGCCATTGGTCGCGACATCGAGTCGATTTACAGCGGCAACAACAAGGGCGTCCTGGCTGACGTTGACCAGCTGCAGCGCATCACCGAGGCCTCCCGCACCTGCGTGCGCGAGTTCGTGAAGGACCGCACCGGCCTGAACGGCCGCATCGGCACCAACTGGATCACCAACCTGCTCAAGTTCACCGGCTTCTACGTGGACCCCTGGGTGCGCGGCCTGCAGAACGGCGAGTTCGCCAGCGCCAACCGCGAGGAGCTGATCACCCTGTTCAACTACCTGGAGTTCTGCCTGACCCAGGTGGTCAAGGACAACGAGCTGGGCGCCCTGGTAGAGGCGCTGAACGGCCAGTACGTCGAGCCCGGCCCCGGCGGTGACCCCATCCGCAACCCCAACGTGCTGCCCACCGGCAAGAACATCCACGCCCTGGACCCTCAGTCGATTCCCACTCAGGCCGCGCTGAAGAGCGCCCGCCTGGTGGTGGACCGCCTGCTGGACCGCGAGCGCGACAACAACGGCGGCAAGTACCCCGAGACCATCGCGCTGGTGCTGTGGGGCACTGACAACATCAAGACCTACGGCGAGTCGCTGGCCCAGGTCATGATGATGGTCGGTGTCAAGCCCGTGGCCGACGCCCTGGGCCGCGTGAACAAGCTGGAGGTGATCCCTCTGGAGGAGCTGGGCCGCCCCCGCGTGGACGTGGTTGTCAACTGCTCGGGTGTGTTCCGCGACCTGTTCGTGAACCAGATGCTGCTGCTGGACCGCGCCATCAAGCTGGCGGCCGAGCAGGACGAGCCCGATGAGATGAACTTCGTGCGCAAGCACGCCAAGCAGCAGGCGGCGGAGCTGGGCCTGCAGAGCCTGCGCGACGCGGCCACCCGTGTGTTCTCCAACAGCTCGGGCTCCTACTCGTCCAACGTCAACCTGGCGGTGGAGAACAGCAGCTGGAGCGACGAGTCGCAGCTGCAGGAGATGTACCTGAAGCGCAAGTCGTACGCCTTCAACTCGGACCGCCCCGGCGCCGGTGGCGAGATGCAGCGCGACGTGTTCGAGACGGCCATGAAGACCGTGGACGTGACCTTCCAGAACCTGGACTCGTCCGAGATCTCGCTGACCGATGTGTCGCACTACTTCGACTCCGACCCCACCAAGCTGGTGGCGTCGCTGCGCAACGACGGCCGCACCCCCAACGCCTACATCGCCGACACCACCACCGCCAACGCGCAGGTCCGCACTCTGGGTGAGACCGTGCGCCTGGACGCCCGCACCAAGCTGCTCAACCCCAAGTGGTACGAGGGCATGCTTGCCTCGGGCTACGAGGGCGTGCGCGAGATCCAGAAGCGCATGACCAACACCATGGGCTGGTCGGCCACCTCGGGCATGGTGGACAACTGGGTGTACGACGAGGCCAACTCGACCTTCATCGAGGATGCGGCCATGGCCGAGCGCCTGATGAACACCAACCCCAACAGCTTCCGCAAGCTGGTGGCCACCTTCCTGGAGGCCAACGGCCGCGGCTACTGGGACGCCAAGCCCGAGCAGCTGGAGCGCCTGCGCCAGCTGTACATGGACGTGGAGGACAAGATTGAGGGCGTCGAATAAGCGGCCTCCCCTTCATGGTAGCACTAGTTGGCGGGTTGTGGTTGGACTAGGCGGCTAGGGTATATACCTAGTAGCGGCGGCTGCGGAGTGGAGGGCTGGCGCCCAGCGCGAGGGCGTGGCCTTTCCTCCTGGACCCGAGAGCGCTCCGCGAGG<br>AGACGGCGAGTGAGATAGGCAGCAGCG</FONT>
-         20         30         40         50         60
-MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDHPFTACNV ATGPRPPMTT FTGGNKGPAK
-         80         90        100        110        120
-QQVSLDLRDE GAGMFTSTSP EMRRVVPDDV KGRVKVKVVY VVLEAQYQSA ISAAVKNINA
-        140        150        160        170        180
-KNSKVCFEVV GYLLEELRDQ KNLDMLKEDV ASANIFIGSL IFIEELAEKI VEAVSPLREK
-        200        210        220        230        240
-LDACLIFPSM PAVMKLNKLG TFSMAQLGQS KSVFSEFIKS ARKNNDNFEE GLLKLVRTLP
-        260        270        280        290        300
-KVLKYLPSDK AQDAKNFVNS LQYWLGGNSD NLENLLLNTV SNYVPALKGV DFSVAEPTAY
-        320        330        340        350        360
-PDVGIWHPLA SGMYEDLKEY LNWYDTRKDM VFAKDAPVIG LVLQRSHLVT GDEGHYSGVV
-        380        390        400        410        420
-AELESRGAKV IPVFAGGLDF SAPVKKFFYD PLGSGRTFVD TVVSLTGFAL VGGPARQDAP
-        440        450        460        470        480
-KAIEALKNLN VPYLVSLPLV FQTTEEWLDS ELGVHPVQVA LQVALPELDG AMEPIVFAGR
-        500        510        520        530        540
-DSNTGKSHSL PDRIASLCAR AVNWANLRKK RNAEKKLAVT VFSFPPDKGN VGTAAYLNVF
-        560        570        580        590        600
-GSIYRVLKNL QREGYDVGAL PPSEEDLIQS VLTQKEAKFN STDLHIAYKM KVDEYQKLCP
-        620        630        640        650        660
-YAEALEENWG KPPGTLNTNG QELLVYGRQY GNVFIGVQPT FGYEGDPMRL LFSKSASPHH
-        680        690        700        710        720
-GFAAYYTFLE KIFKADAVLH FGTHGSLEFM PGKQVGMSGV CYPDSLIGTI PNLYYYAANN
-        740        750        760        770        780
-PSEATIAKRR SYANTISYLT PPAENAGLYK GLKELKELIS SYQGMRESGR AEQICATIIE
-        800        810        820        830        840
-TAKLCNLDRD VTLPDADAKD LTMDMRDSVV GQVYRKLMEI ESRLLPCGLH VVGCPPTAEE
-        860        870        880        890        900
-AVATLVNIAE LDRPDNNPPI KGMPGILARA IGRDIESIYS GNNKGVLADV DQLQRITEAS
-        920        930        940        950        960
-RTCVREFVKD RTGLNGRIGT NWITNLLKFT GFYVDPWVRG LQNGEFASAN REELITLFNY
-        980        990       1000       1010       1020
-LEFCLTQVVK DNELGALVEA LNGQYVEPGP GGDPIRNPNV LPTGKNIHAL DPQSIPTQAA
-       1040       1050       1060       1070       1080
-LKSARLVVDR LLDRERDNNG GKYPETIALV LWGTDNIKTY GESLAQVMMM VGVKPVADAL
-       1100       1110       1120       1130       1140
-GRVNKLEVIP LEELGRPRVD VVVNCSGVFR DLFVNQMLLL DRAIKLAAEQ DEPDEMNFVR
-       1160       1170       1180       1190       1200
-KHAKQQAAEL GLQSLRDAAT RVFSNSSGSY SSNVNLAVEN SSWSDESQLQ EMYLKRKSYA
-       1220       1230       1240       1250       1260
-FNSDRPGAGG EMQRDVFETA MKTVDVTFQN LDSSEISLTD VSHYFDSDPT KLVASLRNDG
-       1280       1290       1300       1310       1320
-RTPNAYIADT TTANAQVRTL GETVRLDART KLLNPKWYEG MLASGYEGVR EIQKRMTNTM
-       1340       1350       1360       1370       1380
-GWSATSGMVD NWVYDEANST FIEDAAMAER LMNTNPNSFR KLVATFLEAN GRGYWDAKPE
-       1400
-QLERLRQLYM DVEDKIEGVE
-References and documentation are available.
+References and documentation are available.
 Please note the modified algorithm for extinction coefficient.
+--------------------------------------------------------------------------------
+Number of amino acids: 1364
-Number of amino acids: 1400
+Molecular weight: 150738.5
-Molecular weight: 154817.9
+Theoretical pI: 5.39
-Theoretical pI: 5.48
+Amino acid composition: Ala (A) 114	  8.4%
+Arg (R)  69	  5.1%
+Asn (N)  79	  5.8%
+Asp (D)  81	  5.9%
+Cys (C)  13	  1.0%
+Gln (Q)  46	  3.4%
+Glu (E)  91	  6.7%
+Gly (G) 100	  7.3%
+His (H)  14	  1.0%
+Ile (I)  51	  3.7%
+Leu (L) 141	 10.3%
+Lys (K)  79	  5.8%
+Met (M)  35	  2.6%
+Phe (F)  50	  3.7%
+Pro (P)  70	  5.1%
+Ser (S)  84	  6.2%
+Thr (T)  69	  5.1%
+Trp (W)  14	  1.0%
+Tyr (Y)  49	  3.6%
+Val (V) 115	  8.4%
+Pyl (O)   0	  0.0%
+Sec (U)   0	  0.0%
-Amino acid composition:
+ (B)   0	  0.0%
-Ala (A) 116 8.3% Arg (R) 71 5.1% Asn (N) 79 5.6% Asp (D) 86 6.1% Cys (C) 13 0.9% Gln (Q) 48 3.4% Glu (E) 92 6.6% Gly (G) 105 7.5% His (H) 21 1.5% Ile (I) 51 3.6% Leu (L) 142 10.1% Lys (K) 80 5.7% Met (M) 38 2.7% Phe (F) 51 3.6% Pro (P) 71 5.1% Ser (S) 86 6.1% Thr (T) 71 5.1% Trp (W) 14 1.0% Tyr (Y) 50 3.6% Val (V) 115 8.2% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
+ (Z)   0	  0.0%
+ (X)   0	  0.0%
-Total number of negatively charged residues (Asp + Glu): 178
+Total number of negatively charged residues (Asp + Glu): 172
-Total number of positively charged residues (Arg + Lys): 151
+Total number of positively charged residues (Arg + Lys): 148
 Atomic composition:
-Carbon      C	      6872
+Carbon      C	      6703
-Hydrogen    H	     10826
+Hydrogen    H	     10581
-Nitrogen    N	      1876
+Nitrogen    N	      1817
-Oxygen      O	      2091
+Oxygen      O	      2036
-Sulfur      S	        51
+Sulfur      S	        48
-Formula: C6872H10826N1876O2091S51
+Formula: C6703H10581N1817O2036S48
-Total number of atoms: 21716
+Total number of atoms: 21185
 Extinction coefficients:
@@ Line 121: / Line 69: @@
 Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.
-Ext. coefficient   152250
+Ext. coefficient   150760
-Abs 0.1% (=1 g/l)   0.983, assuming all pairs of Cys residues form cystines
+Abs 0.1% (=1 g/l)   1.000, assuming all pairs of Cys residues form cystines
-Ext. coefficient   151500
+Ext. coefficient   150010
-Abs 0.1% (=1 g/l)   0.979, assuming all Cys residues are reduced
+Abs 0.1% (=1 g/l)   0.995, assuming all Cys residues are reduced
 Estimated half-life:
@@ Line 140: / Line 88: @@
 Instability index:
-The instability index (II) is computed to be 32.82
+The instability index (II) is computed to be 32.83
 This classifies the protein as stable.
-Aliphatic index: 85.87
+Aliphatic index: 87.71
-Grand average of hydropathicity (GRAVY): -0.292
+Grand average of hydropathicity (GRAVY): -0.260
 ===Source===