Difference between revisions of "Part:BBa K1119004"
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| − | A constitutive Human Elongation Factor-1alpha (EF-1alpha) promoter regulate gene expression in mammalian cells. EF-1alpha promtoer is often useful in conditions where other mammalian constitutive promoters (such as CMV promoter) have diminished activity or have been silenced. The origin of this part is Homo sapiens chromosome 6 genomic contig, GRCh37. p13. We cloned EF-1alpha promoter from | + | A constitutive Human Elongation Factor-1alpha (EF-1alpha) promoter regulate gene expression in mammalian cells. EF-1alpha promtoer is often useful in conditions where other mammalian constitutive promoters (such as CMV promoter) have diminished activity or have been silenced. The origin of this part is Homo sapiens chromosome 6 genomic contig, GRCh37. p13. We cloned EF-1alpha promoter from iDUET101a plasmid(Addgene). |
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Revision as of 08:47, 25 September 2013
Human Elongation Factor-1alpha Promoter
A constitutive Human Elongation Factor-1alpha (EF-1alpha) promoter regulate gene expression in mammalian cells. EF-1alpha promtoer is often useful in conditions where other mammalian constitutive promoters (such as CMV promoter) have diminished activity or have been silenced. The origin of this part is Homo sapiens chromosome 6 genomic contig, GRCh37. p13. We cloned EF-1alpha promoter from iDUET101a plasmid(Addgene).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 84
- 1000COMPATIBLE WITH RFC[1000]