Difference between revisions of "Part:BBa K1062002"
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==='''Usage and Biology'''===
 
==='''Usage and Biology'''===
 
In both of our projects, we used gRNAs and dCas9 as:  
 
In both of our projects, we used gRNAs and dCas9 as:  
−
1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project  
+
 
 +
1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project However, the gRNA for XylE is not used in the conjugation project since we did not use any of the pigments in this project.
 +
 
 
2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.
 
2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.
−
However, the gRNA for GFP is not used in the conjugation project since the green fluorescent protein is to represent the donor strain of the project.
 
  
 
=='''Conjugation Project'''==
 
=='''Conjugation Project'''==
  
−
'''The GFP gRNA was not used in the Conjugation Project'''
+
'''The XylE (middle) gRNA was not used in the Conjugation Project'''
  
 
=='''Synthetic Circuit'''==
 
=='''Synthetic Circuit'''==
  
 
[[File:Synthetic Circuit.jpg]]
 
[[File:Synthetic Circuit.jpg]]
 +
 +
In the Synthetic Circuit, the target is the middle of the XylE gene that would be located in the '''Low Concentration Pathway'''. The purpose of the gRNA is to prevent any possibility of the XylE gene expressing, due to a possible leaky promoter, when the '''High Concentration Pathway''' is being induced.
 +
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K1062002 parameters</partinfo>
 
<partinfo>BBa_K1062002 parameters</partinfo>
 
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Latest revision as of 07:03, 25 September 2013

Guide RNA (gRNA) target for XylE (middle of gene)

This part is a guide RNA (gRNA) that targets the middle of the XylE gene, when bound to Cas9.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

In both of our projects, we used gRNAs and dCas9 as:

1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project However, the gRNA for XylE is not used in the conjugation project since we did not use any of the pigments in this project.

2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.

Conjugation Project

The XylE (middle) gRNA was not used in the Conjugation Project

Synthetic Circuit

Synthetic Circuit.jpg

In the Synthetic Circuit, the target is the middle of the XylE gene that would be located in the Low Concentration Pathway. The purpose of the gRNA is to prevent any possibility of the XylE gene expressing, due to a possible leaky promoter, when the High Concentration Pathway is being induced.