Difference between revisions of "Part:BBa C0020:Design"

Revision as of 17:22, 17 July 2006

CheY "tumbling" chemotaxis coding sequence

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Changes from the NCBI sequence: A BioBricks restriction site for PstI was at position 260. As a result, the codon starting at position 262 was changed from "gca" to "gcg".

The terminating codon at the end, "tga", was changed to "taataa" as per BioBrick regulations as told to us by Randy Rettberg.

Source

Organism: NCBI - Escherichia coli K12 MG1655 (NC_000913)
Paper: P Matsumura, J J Rydel, R Linzmeier and D Vacante. Journal of Bacteriology. 1984 "Overexpression and sequence of the Escherichia coli cheY gene and biochemical activities of the CheY protein." [http://jb.asm.org/cgi/content/abstract/160/1/36?vol=160&fp=36&view=abstract link]

References

UniProt data available [http://www.pir.uniprot.org/cgi-bin/upEntry?id=CHEY_ECOLI here]
SwissProt data available [http://ca.expasy.org/cgi-bin/niceprot.pl?CHEY_ECOLI here]
NCBI sequence data [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=946393 available here]

@@ Line 16: / Line 16: @@
 ===Source===
-NCBI - Escherichia coli K12 MG1655 (NC_000913)
+*Organism: NCBI - Escherichia coli K12 MG1655 (NC_000913)
+*Paper: P Matsumura, J J Rydel, R Linzmeier and D Vacante.  Journal of Bacteriology. 1984 "Overexpression and sequence of the Escherichia coli cheY gene and biochemical activities of the CheY protein." [http://jb.asm.org/cgi/content/abstract/160/1/36?vol=160&fp=36&view=abstract link]
 ===References===