Difference between revisions of "Part:BBa C0020:Design"

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===References===
 
===References===
  
 +
* UniProt data available [http://www.pir.uniprot.org/cgi-bin/upEntry?id=CHEY_ECOLI here]
 +
* SwissProt data available [http://ca.expasy.org/cgi-bin/niceprot.pl?CHEY_ECOLI here]
 
* NCBI sequence data [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=946393  available here]
 
* NCBI sequence data [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=946393  available here]

Revision as of 17:19, 17 July 2006


CheY "tumbling" chemotaxis coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Changes from the NCBI sequence: A BioBricks restriction site for PstI was at position 260. As a result, the codon starting at position 262 was changed from "gca" to "gcg".

The terminating codon at the end, "tga", was changed to "taataa" as per BioBrick regulations as told to us by Randy Rettberg.


Source

NCBI - Escherichia coli K12 MG1655 (NC_000913)

References

  • UniProt data available [http://www.pir.uniprot.org/cgi-bin/upEntry?id=CHEY_ECOLI here]
  • SwissProt data available [http://ca.expasy.org/cgi-bin/niceprot.pl?CHEY_ECOLI here]
  • NCBI sequence data [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=946393 available here]