Difference between revisions of "Part:BBa J176001"
 
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<partinfo>BBa_J176001 short</partinfo>
 
<partinfo>BBa_J176001 short</partinfo>
  
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Polycomb chromodomain (PCD) from the zebrafish CBX2 protein (a.a. 2-64).
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Polycomb chromodomain from the Drosophila (fruit fly) Pc protein (a.a. 16-78).<br>
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Alias: KAH03
  
  
 
===Usage and Biology===
 
===Usage and Biology===
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[[Image:Haynes_Pc1_PDB1PDQ.png|thumb|250px|'''Drosophila PCD of Pc1''' (orange) Crystal structure reported by Fischle et al. 2011. Grey = H3 histone tail; Ball and stick = trimethylated lysine 27 ]]
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* Protein Data Bank ID 1PDQ
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* Mammalian expression vector required
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* Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression<br>
  
 
See the human variant <partinfo>BBa_J176000</partinfo> for more details on the biology of the Polycomb chromodomain.
 
See the human variant <partinfo>BBa_J176000</partinfo> for more details on the biology of the Polycomb chromodomain.
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<br><br><br>
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REFERENCES:
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# Fischle, W, Wang, Y, Jacobs, SA, Kim, Y, Allis, CD, Khorasanizadeh, S. (2003) Molecular basis for the discrimination of repressive methyl-lysine marks in histone H3 by Polycomb and HP1 chromodomains. Genes Dev. 17:1870-1881.
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# Min, J, Zhang, Y, Xu, RM. (2003) Structural basis for the specific binding of Polycomb chromodomain to histone H3 methylated at Lys 27. Genes Dev. 17:1823-1828.
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# Haynes, KA, Silver, PA. (2011) Synthetic reversal of epigenetic silencing. J Biol Chem. 286:27176-27182.
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<br><br><br><br><br><br><br><br>
  
 
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Latest revision as of 03:11, 25 September 2013

flyPCD

Polycomb chromodomain from the Drosophila (fruit fly) Pc protein (a.a. 16-78).
Alias: KAH03


Usage and Biology

Drosophila PCD of Pc1 (orange) Crystal structure reported by Fischle et al. 2011. Grey = H3 histone tail; Ball and stick = trimethylated lysine 27
  • Protein Data Bank ID 1PDQ
  • Mammalian expression vector required
  • Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression

See the human variant BBa_J176000 for more details on the biology of the Polycomb chromodomain.




REFERENCES:

  1. Fischle, W, Wang, Y, Jacobs, SA, Kim, Y, Allis, CD, Khorasanizadeh, S. (2003) Molecular basis for the discrimination of repressive methyl-lysine marks in histone H3 by Polycomb and HP1 chromodomains. Genes Dev. 17:1870-1881.
  2. Min, J, Zhang, Y, Xu, RM. (2003) Structural basis for the specific binding of Polycomb chromodomain to histone H3 methylated at Lys 27. Genes Dev. 17:1823-1828.
  3. Haynes, KA, Silver, PA. (2011) Synthetic reversal of epigenetic silencing. J Biol Chem. 286:27176-27182.









Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]