Difference between revisions of "Part:BBa J176002"

Latest revision as of 03:04, 25 September 2013

fshPCD

Polycomb chromodomain (PCD) from the zebrafish CBX2 protein (a.a. 2-64). Other names: KAH04

Chassis: mammalian cells
Mammalian expression vector required
Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression

See the human variant BBa_J176000 for more details on the biology of the Polycomb chromodomain.

Sequence and Features

Assembly Compatibility:

@@ Line 2: / Line 2: @@
 <partinfo>BBa_J176002 short</partinfo>
-Polycomb chromodomain from the Zebrafish CBX2 protein<br>
+Polycomb chromodomain (PCD) from the zebrafish CBX2 protein (a.a. 2-64).
-Alias: KAH04
+Other names: KAH04
 ===Usage and Biology===
-[[Image:Haynes_Pc1_PDB1PDQ.png|thumb|250px|'''Drosophila PCD of Pc1''' (orange) Crystal structure reported by Fischle et al. 2011. Grey = H3 histone tail; Ball and stick = trimethylated lysine 27 ]]
+Usage and Biology
+* Chassis: mammalian cells
-* Protein Data Bank ID 1PDQ
 * Mammalian expression vector required
-* Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression<br>
+* Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression
 See the human variant <partinfo>BBa_J176000</partinfo> for more details on the biology of the Polycomb chromodomain.
-<br><br><br>
-REFERENCES:
-# Fischle, W, Wang, Y, Jacobs, SA, Kim, Y, Allis, CD, Khorasanizadeh, S. (2003) Molecular basis for the discrimination of repressive methyl-lysine marks in histone H3 by Polycomb and HP1 chromodomains. Genes Dev. 17:1870-1881.
-# Min, J, Zhang, Y, Xu, RM. (2003) Structural basis for the specific binding of Polycomb chromodomain to histone H3 methylated at Lys 27. Genes Dev. 17:1823-1828.
-# Haynes, KA, Silver, PA. (2011) Synthetic reversal of epigenetic silencing. J Biol Chem. 286:27176-27182.
-<br><br><br><br><br><br><br><br>