Difference between revisions of "Part:BBa J176002"

Revision as of 02:28, 25 September 2013

fshPCD

Polycomb chromodomain from the Zebrafish CBX2 protein
Alias: KAH04

Usage and Biology

Drosophila PCD of Pc1 (orange) Crystal structure reported by Fischle et al. 2011. Grey = H3 histone tail; Ball and stick = trimethylated lysine 27

• Protein Data Bank ID 1PDQ
• Mammalian expression vector required
• Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression
  

See the human variant BBa_J176000 for more details on the biology of the Polycomb chromodomain.

REFERENCES:

1. Fischle, W, Wang, Y, Jacobs, SA, Kim, Y, Allis, CD, Khorasanizadeh, S. (2003) Molecular basis for the discrimination of repressive methyl-lysine marks in histone H3 by Polycomb and HP1 chromodomains. Genes Dev. 17:1870-1881.
2. Min, J, Zhang, Y, Xu, RM. (2003) Structural basis for the specific binding of Polycomb chromodomain to histone H3 methylated at Lys 27. Genes Dev. 17:1823-1828.
3. Haynes, KA, Silver, PA. (2011) Synthetic reversal of epigenetic silencing. J Biol Chem. 286:27176-27182.

Sequence and Features