Difference between revisions of "Part:BBa K1114108:Design"

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Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.  See <a href=”https://parts.igem.org/Part:BBa_K783055“>BBa_K783055</a>.
 
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.  See <a href=”https://parts.igem.org/Part:BBa_K783055“>BBa_K783055</a>.
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===Source===
 
===Source===
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This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by <a href= “http://www.nature.com/nmeth/journal/v10/n4/full/nmeth.2404.html”?>Mutalik, et al.</a>
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This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD8 part off of a paper published by <a href= "http://www.nature.com/nmeth/journal/v10/n4/full/nmeth.2404.html">Mutalik, et al.</a>
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===References===
 
===References===
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<a href= “http://www.nature.com/nmeth/journal/v10/n4/full/nmeth.2404.html”?>Mutalik, et al.</a>
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<a href= "http://www.nature.com/nmeth/journal/v10/n4/full/nmeth.2404.html">Mutalik, et al.</a>
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Latest revision as of 19:50, 24 September 2013

Bicistronic design RBS 8, MoClo Format with BC fusion sites



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Design Notes

This is the MoClo formatted part of a bicistronic design ribosomal binding site 8 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.



Source

This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD8 part off of a paper published by Mutalik, et al.

References

Mutalik, et al.