Difference between revisions of "Part:BBa K1045014"

Revision as of 19:18, 24 September 2013

Promoter reverse - Promoter - DarR operator - GFP generator BBa_E0240

This part consists of BBa_K1045011 (a weak, inverse promoter) placed upstream of BBa_K1045013 (a GFP transcription unit harboring the DarR operator downstream of a strong promoter). Since BBa_K1045011 has an inverse orientation compared to BBa_K10450013, the composite part BBa_K1045014 could be used to clone an inverse coding sequence with an inverse RBS behind the inverse promoter BBa_K1045011 yielding a vector from which both, GFP and the gene of interest might be expressed. Additionally, if the repressor DarR is present, GFP expression might be controlled by DarR. Note that it might be useful to place a terminator behind the inverse coding sequence to ensure that expression of the inverse coding sequence does not influence the expression of GFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1
Illegal NheI site found at 24
Illegal NheI site found at 104
Illegal NheI site found at 127
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 55
Illegal AgeI site found at 963
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 825

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1045014 short</partinfo>
-This part consists of BBa_K1045011 (a weak, inverse promoter) placed upstream of BBa_K1045013 (a GFP transcription unit harboring the DarR operator downstream of a strong promoter). Since BBa_K1045011 has an inverse orientation compared to BBa_K10450013, the composite part BBa_K1045014 could be used to clone an inverse coding sequence with an inverse RBS behind the inverse promoter BBa_K1045011 yielding a vector from which both, GFP and the gene of interest might be expressed. Additionally, if the repressor DarR is present, GFP expression might be controlled by DarR. Note that it might be useful to place a terminator behind the inverse coding sequence to ensure that expression of the terminator does not influence the expression of GFP.
+This part consists of BBa_K1045011 (a weak, inverse promoter) placed upstream of BBa_K1045013 (a GFP transcription unit harboring the DarR operator downstream of a strong promoter). Since BBa_K1045011 has an inverse orientation compared to BBa_K10450013, the composite part BBa_K1045014 could be used to clone an inverse coding sequence with an inverse RBS behind the inverse promoter BBa_K1045011 yielding a vector from which both, GFP and the gene of interest might be expressed. Additionally, if the repressor DarR is present, GFP expression might be controlled by DarR. Note that it might be useful to place a terminator behind the inverse coding sequence to ensure that expression of the inverse coding sequence does not influence the expression of GFP.
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