Difference between revisions of "Part:BBa K639003:Experience"
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| − | | [[File:OD_stress.png|thumbnail|right|400px|Cell growth over 6h with IPTG induction. mCherry production is induced by the stress pathway and detection of ppGpps. In order to bypass this, we induced with IPTG which inhibits LacI, resulting in mCherry expression.]] | + | | [[http://2013.igem.org/File:OD_stress.png|thumbnail|right|400px|Cell growth over 6h with IPTG induction. mCherry production is induced by the stress pathway and detection of ppGpps. In order to bypass this, we induced with IPTG which inhibits LacI, resulting in mCherry expression.]] |
| [[File:Fluorescence_stress.png|thumbnail|right|400px|Fluorescence of the cells under IPTG induction over a 6h period.]] | | [[File:Fluorescence_stress.png|thumbnail|right|400px|Fluorescence of the cells under IPTG induction over a 6h period.]] | ||
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Revision as of 18:51, 24 September 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K639003
User Reviews
UNIQ4f44dbb0790e36f4-partinfo-00000000-QINU UNIQ4f44dbb0790e36f4-partinfo-00000001-QINU
Imperial iGEM 2013
We intended on using BBa_K639003 to detect whether our cells were stressed when grown in mixed waste, mixed waste conditioned media or in the presence of plastic toxic byproducts. However, as the data below shows, this mCherry biosensor is very leaky, with clear expression in an un-induced or non-stressed state. However based upon our characterisation data the biosensor does induce increased mCherry expression as expected and therefore we recommend future teams try to improve the part by reducing its leakyness.
We did however find the sensor to be useful to our project as a marker of our chassis in extended waste toxicity assays. The data is included below.
