Difference between revisions of "Part:BBa K1114702"

Revision as of 18:08, 24 September 2013

BioBrick adaptation of MoClo Level 1 Reporter

This is a BioBrick adaptation of a Level 1 MoClo device that constitutively expresses RFP. It contains the Level 0 parts J23104_AB, BCD1_BC, E0040m_CD, and B0015_DE within the pSB1C3 backbone. In the given sequence the internal fusion sites are incorrectly duplicated. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.

This part contains a bicistronic design (BCD) element as the 5' UTR regulatory part from Mutalik et al., 2013. From the library of BCDs, this devices contains a MoClo version of BCD1 (BioFAB # apFAB681) and we obtained the sequence data for this part from the BioFAB.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 11
Illegal NheI site found at 34
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 694
Illegal AgeI site found at 806
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
+<br><br>
+This part contains a bicistronic design (BCD) element as the 5' UTR regulatory part from <a href="http://www.nature.com/nmeth/journal/v10/n4/abs/nmeth.2404.html">Mutalik et al., 2013</a>. From the library of BCDs, this devices contains a MoClo version of BCD1 (BioFAB # apFAB681) and we obtained the sequence data for this part from the <a href="http://biofab.org/data">BioFAB</a>.
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