Difference between revisions of "Part:BBa K1073029"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The expression rate of the genes downstream of the promoter region can be tuned by the concentration of N-3-oxo-hexanoyl-HSL in the media. In this case the expression of beta lactamase can be induced by adding N-3-oxo-hexanoyl-HSL to the culture broth. | ||
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Revision as of 18:01, 24 September 2013
Inducible ampicillin resistance cassette + LuxR expression cassette
In complex with the autoinducer molecule N-3-oxo-hexanoyl-HSL, the transcription regulator LuxR binds to specific sequences in the promoter region and enables transcription of ampR. This construct provides all the parts required to express the gene downstream of the inducible promoter. Only LuxI, the synthase producing the autoinducer molecule N-3-oxo-hexanoyl-HSL is missing in the construct. However, N-3-oxo-hexanoyl-HSL can be taken up from the media.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1098
Illegal NheI site found at 1121 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]