Difference between revisions of "Part:BBa K1114500"

Revision as of 17:33, 24 September 2013

MoClo Level 1 RFP reporter with AE fusion sites

pJ04B1Rm_AE

This is a MoClo Level 1 reporting device containing the Level 0 parts J23104_AB, BCD1_BC, E1010m_CD, and B0015_DE within the DVL1_AE backbone. The flanking fusion sites are A on the 5' side and E on the 3' side of the insert. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.

This part contains a bicistronic design (BCD) element as the 5' UTR regulatory part from Mutalik et al., 2013. From the library of BCDs from Mutalik, this contains BCD2 and we obtained the sequence data for this part from the BioFAB.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 11
Illegal NheI site found at 34
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 694
Illegal AgeI site found at 806
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
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-This part contains a bicistronic design (BCD) element as the 5' UTR regulatory part from <a href="http://www.nature.com/nmeth/journal/v10/n4/abs/nmeth.2404.html">Mutalik et al., 2013</a>.
+This part contains a bicistronic design (BCD) element as the 5' UTR regulatory part from <a href="http://www.nature.com/nmeth/journal/v10/n4/abs/nmeth.2404.html">Mutalik et al., 2013</a>. From the library of BCDs from Mutalik, this contains BCD2 and we obtained the sequence data for this part from the <a href="http://biofab.org/data">BioFAB</a>.
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