Difference between revisions of "Part:BBa K1065305:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1065305 short</partinfo> | <partinfo>BBa_K1065305 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | Part <partinfo>Bba_k952003</partinfo> was missing an RBS before the amilGFP CDS, thus it doesn't work. We | + | Part <partinfo>Bba_k952003</partinfo> was missing an RBS before the amilGFP CDS, thus it doesn't work. We successfully inserted the RBS by mutagenesis |
===Source=== | ===Source=== | ||
− | + | The part consist of: <BR> | |
+ | Bba_K952003: the entire blue light sensing device with fluorescent output.<BR> | ||
+ | RBS sequence | ||
+ | ===References=== | ||
− | + | 1) Moglich A, Ayers RA and Moffat K. (2009) Design and Signaling Mechanism of Light-Regulated Histidine Kinases. J. Mol. Bio. 385, 5, 1433-1444.<BR> | |
+ | 2)Ohlendorf, R., Vidavski, R.R., Eldar, A., Moffat, K. & Möglich, A.(2012). From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression. J. Mol. Biol., 416: 534: 542 |
Revision as of 17:17, 24 September 2013
Blue light sensor without inverter for the production of amilGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 562
Illegal NgoMIV site found at 634
Illegal NgoMIV site found at 724
Illegal NgoMIV site found at 742
Illegal NgoMIV site found at 1254
Illegal NgoMIV site found at 1547
Illegal NgoMIV site found at 1641
Illegal AgeI site found at 276
Illegal AgeI site found at 1422 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1311
Illegal BsaI.rc site found at 175
Design Notes
Part BBa_K952003 was missing an RBS before the amilGFP CDS, thus it doesn't work. We successfully inserted the RBS by mutagenesis
Source
The part consist of:
Bba_K952003: the entire blue light sensing device with fluorescent output.
RBS sequence
References
1) Moglich A, Ayers RA and Moffat K. (2009) Design and Signaling Mechanism of Light-Regulated Histidine Kinases. J. Mol. Bio. 385, 5, 1433-1444.
2)Ohlendorf, R., Vidavski, R.R., Eldar, A., Moffat, K. & Möglich, A.(2012). From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression. J. Mol. Biol., 416: 534: 542