Difference between revisions of "Part:BBa K1073028"
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<partinfo>BBa_K1073028 short</partinfo>
 
<partinfo>BBa_K1073028 short</partinfo>
  
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In complex with the autoinducer molecule N-butyryl-HSL, the transcription regulator RhlR binds to specific sequences in the promoter region and enables transcription of ''ampR''. This construct provides all the parts required to express the gene Downstream of the inducible promoter. Only RhlI, the synthase producing the inducer molecule N-butyryl-HSL is missing. N-buturyl-HSL can be taken up from the media.  
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In complex with the autoinducer molecule N-butyryl-HSL, the transcription regulator RhlR binds to specific sequences in the promoter region and enables transcription of ''ampR''. This construct provides all the parts required to express the gene Downstream of the inducible promoter. Only RhlI, the synthase producing the autoinducer molecule N-butyryl-HSL is missing in the construct. However, N-buturyl-HSL can be taken up from the media.  
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 15:48, 24 September 2013

Inducible ampicillin resistance cassette + RhlR expression cassette

In complex with the autoinducer molecule N-butyryl-HSL, the transcription regulator RhlR binds to specific sequences in the promoter region and enables transcription of ampR. This construct provides all the parts required to express the gene Downstream of the inducible promoter. Only RhlI, the synthase producing the autoinducer molecule N-butyryl-HSL is missing in the construct. However, N-buturyl-HSL can be taken up from the media.

Usage and Biology

The expression rate of the genes downstream of the promoter region can be tuned by the concentration of N-buturyl-HSL in the media. In this case the expression of beta lactamase can be induced by adding N-buturyl-HSL to the culture broth.

Note that due to the low level leakiness of the promoter there is background activity of the beta lactamase even when N-buturyl-HSL is not present.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1096
    Illegal NheI site found at 1119
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1391
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1866