Difference between revisions of "Part:BBa K1139021"

Revision as of 14:26, 24 September 2013

Plux-M13-Plac-GFP on pSB3

M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell. This biobrick is extracted from M13mp18 phage vector by PCR. It inclueds 11 ORFs, M13 origin, a packaging sequence and lac promoter. The promoter on the upstream of g2(gene 2) is altered to lux promoter. A phage particle is formed only when the host cell receives AHL signal(3OC6HSL, C6) because g2p(gene 2 protein) is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle.

As a reporter, GFP is inserted on the downstream of the lac promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 163
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 6089
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 7353

@@ Line 3: / Line 3: @@
 M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.
-This biobrick is extracted from M13mp18 phage vector by PCR. It inclueds 11 ORFs, M13 origin, a packaging sequence and Lac promoter. The promoter on the upstream of g2(gene 2) is altered to <i>lux</i> promoter.
+This biobrick is extracted from M13mp18 phage vector by PCR. It inclueds 11 ORFs, M13 origin, a packaging sequence and <i>lac</i> promoter. The promoter on the upstream of g2(gene 2) is altered to <i>lux</i> promoter.
 A phage particle is formed only when the host cell receives AHL signal(3OC6HSL, C6) because g2p(gene 2 protein) is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle.