Difference between revisions of "Part:BBa K1054011"
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<partinfo>BBa_K1054011 short</partinfo> | <partinfo>BBa_K1054011 short</partinfo> | ||
| − | BLF2 with | + | BLF2 is the C-terminal of ampicillin resistance gene TEM-1 β-lactamase. It consists of β-lactamase amino acid 198-290. It functions together with the C-terminal of β-lactamase (BLF1, [https://parts.igem.org/Part:BBa_K1054010 BBa_K1054010]), as when they dimerize they possess ampicillinase activity. [http://www.nature.com/nbt/journal/v20/n6/full/nbt0602-619.html [1]] |
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Latest revision as of 13:49, 24 September 2013
BLF2 with linker
BLF2 is the C-terminal of ampicillin resistance gene TEM-1 β-lactamase. It consists of β-lactamase amino acid 198-290. It functions together with the C-terminal of β-lactamase (BLF1, BBa_K1054010), as when they dimerize they possess ampicillinase activity. [http://www.nature.com/nbt/journal/v20/n6/full/nbt0602-619.html [1]]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 131