Difference between revisions of "Part:BBa K1073020"

 
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<partinfo>BBa_K1073020 short</partinfo>
 
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The chromoprotein aeblue is constitutively expressed and can be used for the marking of cells for easier differentiation.
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The device consists of a strong promoter (BBa_J23100) combined with RBS (BBa_B0032) and the encoding region of th chromoprotein aeBlue. This results in a constitutively high expression of aeBlue.  
  
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===Usage and Biology===
 
===Usage and Biology===
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During cultivation the chromoprotein aeBlue is expressed and stored intracellular. The blue color of the protein can be seen by naked eye, thus you can use the chromoprotein as a selection marker and seperate cells easily.
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Revision as of 08:26, 24 September 2013

Constitutively expressed chromoprotein aeBlue

The device consists of a strong promoter (BBa_J23100) combined with RBS (BBa_B0032) and the encoding region of th chromoprotein aeBlue. This results in a constitutively high expression of aeBlue.

Usage and Biology

During cultivation the chromoprotein aeBlue is expressed and stored intracellular. The blue color of the protein can be seen by naked eye, thus you can use the chromoprotein as a selection marker and seperate cells easily.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]