Difference between revisions of "Part:BBa K1049002:Experience"
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===Applications of BBa_K1049002=== | ===Applications of BBa_K1049002=== | ||
Our team KIT-Kyoto 2013 constructed this part for the purpose of measurement. T7 promoter is an IPTG-inducible promoter. We added 20uL IPTG (100mM) to our genetically modified E.coli after cultivation at 28 and 37 degree C. 2 hours after, we extracted soluble proteins from it by using FastBreak™ Cell Lysis Reagent and did SDS-polyacrylamide gel electrophoresis. | Our team KIT-Kyoto 2013 constructed this part for the purpose of measurement. T7 promoter is an IPTG-inducible promoter. We added 20uL IPTG (100mM) to our genetically modified E.coli after cultivation at 28 and 37 degree C. 2 hours after, we extracted soluble proteins from it by using FastBreak™ Cell Lysis Reagent and did SDS-polyacrylamide gel electrophoresis. | ||
| − | [[File: | + | [[File:KIT2013SDS.png|500px|thumb|left|Figure.1 SDS-PAGE Starting from the left, marker, ATF2 at 28 degree C, ATF2 + IPTG at 28 degree C, marker, ATF2 at 37 degree C, ATF2 + IPTG at 37 degree C]] |
ATF2 gene encodes AATase, which is about 62kDa. The consumption of protein marker is like this. | ATF2 gene encodes AATase, which is about 62kDa. The consumption of protein marker is like this. | ||
Revision as of 01:46, 24 September 2013
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1049002
Our team KIT-Kyoto 2013 constructed this part for the purpose of measurement. T7 promoter is an IPTG-inducible promoter. We added 20uL IPTG (100mM) to our genetically modified E.coli after cultivation at 28 and 37 degree C. 2 hours after, we extracted soluble proteins from it by using FastBreak™ Cell Lysis Reagent and did SDS-polyacrylamide gel electrophoresis.
ATF2 gene encodes AATase, which is about 62kDa. The consumption of protein marker is like this.
Myosin 200kDa β‐Galactosidase 120kDa Bovine Serum Albumin 95kDa Glutamine dehydrogenase 68kDa Ovalbumin 50kDa Carbonic Anhydrase 36kDa Myoglobin 27kDa Lysozyme 20kDa Aprotinin 10kDa
You can find the band at lanes which are added IPTG just beneath the band of 68kDa.
User Reviews
UNIQ0408496f2636209b-partinfo-00000000-QINU UNIQ0408496f2636209b-partinfo-00000001-QINU