Difference between revisions of "Part:BBa K1067003"

Revision as of 20:32, 23 September 2013

Ammonium monooxygenase from Nitrosomonas europaea

Ammonia monooxygenase (AMO) from Nitrosomonas europaea converts ammonia to hydroxylamine; NH₃ + O₂ + cycAX_red → NH₂OH + H₂O cycAX_ox

The whole operon containing amoA1, amoB1, amoC1 http://biocyc.org/NEUR228410/NEW-IMAGE?type=OPERON-IN-CHROM-BROWSER&object=TUJNO-524 Biocyc was extracted by colony PCR on a N. europaea culture.

Usage and Biology

AMO is and integral membrane protein composed of 3 subunits (amoA, amoB, amoC) and is first step in the enzymatic conversion of ammonia to nitrite in the nitrifying bacteria genus. The protein works in close relationship with cycA, cycX and HAO in this process. HAO is providing 2 free electrons that are picked up and delivered to AMO by the cytochromes cycA and cycX. The electron from HAO is what makes AMO's work possible.

This part is experienced to slow down growth rates of E.coli substantially; likely because this is a heterologous expressed membrane protein and thereby disrupts E.coli membrane.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 263
Illegal BamHI site found at 306
Illegal BamHI site found at 2510
Illegal BamHI site found at 2822
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2028
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1067003 short</partinfo>
-Ammonia monooxygenase (AMO) from ''Nitrosomonas europaea'' converts ammonia to hydroxylamine; NH<sub>3</sub> + O<sub>2</sub> &rarr; NH<sub>2</sub>OH + H<sub>2</sub>O
+Ammonia monooxygenase (AMO) from ''Nitrosomonas europaea'' converts ammonia to hydroxylamine; NH<sub>3</sub> + O<sub>2</sub> + cycAX<sub>red</sub> &rarr; NH<sub>2</sub>OH + H<sub>2</sub>O cycAX<sub>ox</sub>
 The whole operon containing amoA1, amoB1, amoC1 [[http://biocyc.org/NEUR228410/NEW-IMAGE?type=OPERON-IN-CHROM-BROWSER&object=TUJNO-524 Biocyc]] was extracted by colony PCR on a ''N. europaea'' culture.