Difference between revisions of "Part:BBa K1217003"

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==Usage and Biology==
 
==Usage and Biology==
  
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===Expression of ppk1 enzyme===
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==='''Expression of ppk1 enzyme'''===
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[[File:HKU13_KOppkSDS.png|600px|thumb|none|alt text]]
  
 
Testing
 
Testing

Revision as of 19:44, 23 September 2013

pT7-ppk1(K.oralis)

Polyphosphate kinase (ppk1) (E.C. 2.7.4.1) catalyzes the formation of polyphosphate from ATP. In BBa_K1217003, ppk1 was under the control of T7 promotor. Under iptg induction, over-expression of ppk1 can reduce the phosphate level in the medium by its incorporation into polyphosphate synthesis inside the bacteria.

Usage and Biology

Expression of ppk1 enzyme

alt text

Testing

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 309