Difference between revisions of "Part:BBa K1067001"

 
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__NOTOC__
 
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<partinfo>BBa_K1067001 short</partinfo>
 
<partinfo>BBa_K1067001 short</partinfo>
  
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This biobrick is a part composed of GFP SF exported to the periplasm by twin-arginine translocation (TAT) signal peptide. In addition this is transcriptionally fused to a RFP with RBS associated.
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===Usage and Biology===
 
===Usage and Biology===
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The twin-arginine translocation
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The ration of expression between GFP SF and RFP can be tuned by switching out the RBS sequence upstream GFP SF. 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1067001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1067001 SequenceAndFeatures</partinfo>

Revision as of 19:22, 23 September 2013

Twin-arginine translocation reporter

This biobrick is a part composed of GFP SF exported to the periplasm by twin-arginine translocation (TAT) signal peptide. In addition this is transcriptionally fused to a RFP with RBS associated.


Usage and Biology

The twin-arginine translocation

The ration of expression between GFP SF and RFP can be tuned by switching out the RBS sequence upstream GFP SF.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1225
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1225
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1225
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1225
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 149