Difference between revisions of "Part:BBa K1073013"

 
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<partinfo>BBa_K1073013 short</partinfo>
 
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The promoter for this beta-lactonase is induced by N-(3-oxododecanoyl)-homoserine lactone. Therefore the expression of the ampicillin beta-lactonase is inducable by Las inducer N-(3-oxododecanoyl)-homoserine lactone.
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This device is a combination of BBa_K1073003 and BB_K1073000 and mediates a inducible ampicillin resistance. It includes the inducible promoter of the las quorum sensing system of ''Pseudomonas aeruginosa'' followed by a RBS and the ampicillin resistance gene coding for beta-lactamase.  
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===Usage and Biology===
 
===Usage and Biology===
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The promoter for this beta-lactonase is induced by N-(3-oxododecanoyl)-homoserine lactone. Therefore the expression of the ampicillin beta-lactonase is inducable by Las inducer N-(3-oxododecanoyl)-homoserine lactone.
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The promoter is activated in the presence of LasR in combination with the autoinducer molecule N-3-oxododecanoyl-homoserine lactone produced by LasI. The expression of the beta-lactamase can therefore be induced and tuned by supplying LasR and N-3-oxododecanoyl-HSL.
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Note that the promoter is leaking to a certain degree when not activated. For further information and experience on this device see BBa_K1073034.
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Revision as of 18:52, 23 September 2013

Inducible ampicillin resistance cassette (regulated by LasR + N-3-oxododecanoyl-HSL)

This device is a combination of BBa_K1073003 and BB_K1073000 and mediates a inducible ampicillin resistance. It includes the inducible promoter of the las quorum sensing system of Pseudomonas aeruginosa followed by a RBS and the ampicillin resistance gene coding for beta-lactamase.


Usage and Biology

The promoter for this beta-lactonase is induced by N-(3-oxododecanoyl)-homoserine lactone. Therefore the expression of the ampicillin beta-lactonase is inducable by Las inducer N-(3-oxododecanoyl)-homoserine lactone.

The promoter is activated in the presence of LasR in combination with the autoinducer molecule N-3-oxododecanoyl-homoserine lactone produced by LasI. The expression of the beta-lactamase can therefore be induced and tuned by supplying LasR and N-3-oxododecanoyl-HSL.

Note that the promoter is leaking to a certain degree when not activated. For further information and experience on this device see BBa_K1073034.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]